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Mouse Anti-EN1 Recombinant Antibody (3G9) (CBMAB-1141-YC)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
3G9
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 136-172 within an internal region of human EN-1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Epitope
Located in aa 266-392, partial
More Infomation

Target

Full Name
EN1
Introduction
The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. In mouse, mutations in En1 and En2, produced different developmental defects that frequently are lethal. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments.
Entrez Gene ID
Human2019
Mouse13798
Rat685360
UniProt ID
HumanQ05925
MouseP09065
RatA0A0G2JT82
Alternative Names
homeobox protein engrailed-1; engrailed homolog 1; homeobox protein en-1; hu-En-1
Biological Process
Adult locomotory behavior Source: Ensembl
Anatomical structure morphogenesis Source: ProtInc
Cerebellum development Source: Ensembl
Dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Dorsal/ventral pattern formation Source: Ensembl
Drinking behavior Source: Ensembl
Embryonic brain development Source: Ensembl
Embryonic forelimb morphogenesis Source: Ensembl
Midbrain development Source: Ensembl
Midbrain-hindbrain boundary development Source: Ensembl
Motor learning Source: Ensembl
Multicellular organism growth Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Neuron development Source: Ensembl
Neuron differentiation Source: GO_Central
Pigmentation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Proximal/distal pattern formation Source: Ensembl
Rregulation of transcription by RNA polymerase II Source: GO_Central
Response to cocaine Source: Ensembl
Skeletal system development Source: ProtInc
Social behavior Source: Ensembl
Cellular Location
Nucleus

Yang, L., Li, X., Luo, Y., Yang, T., Wang, H., Shi, L., ... & Xie, W. (2021). Weighted gene co‑expression network analysis of the association between upregulated AMD1, EN1 and VGLL1 and the progression and poor prognosis of breast cancer. Experimental and Therapeutic Medicine, 22(3), 1-13.

Baba, S., Akashi, T., Kayamori, K., Ohuchi, T., Ogawa, I., Kubota, N., ... & Ikeda, T. (2021). Homeobox transcription factor engrailed homeobox 1 is a possible diagnostic marker for adenoid cystic carcinoma and polymorphous adenocarcinoma. Pathology International, 71(2), 113-123.

Györfi, A. H., Matei, A. E., Fuchs, M., Liang, C., Rigau, A. R., Hong, X., ... & Distler, J. H. (2021). Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation. Journal of Experimental Medicine, 218(9), e20201916.

Miura, K., Akashi, T., Namiki, T., Hishima, T., Bae, Y., Sakurai, U., ... & Eishi, Y. (2020). Engrailed homeobox 1 and cytokeratin 19 are independent diagnostic markers of eccrine porocarcinoma and distinguish it from squamous cell carcinoma. American Journal of Clinical Pathology, 154(4), 499-509.

Zhu, J., & Zhang, Y. Q. (2019). Engrailed 1 overexpression as a potential prognostic marker in Lower Grade Glioma. PeerJ, 7, e7414.

Gandhi, N. S., Blancafort, P., & Mancera, R. L. (2018). Atomistic molecular dynamics simulations of bioactive engrailed 1 interference peptides (EN1-iPeps). Oncotarget, 9(32), 22383.

Miura, K., Akashi, T., Ando, N., Ayabe, S., Kayamori, K., Namiki, T., & Eishi, Y. (2018). Homeobox transcriptional factor engrailed homeobox 1 is expressed specifically in normal and neoplastic sweat gland cells. Histopathology, 72(7), 1199-1208.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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