Rabbit Anti-EN1 Recombinant Antibody (4G11) (CBMAB-0227-CN)

Name: Rabbit Anti-EN1 Recombinant Antibody (4G11)
SKU: CBMAB-0227-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

4G11

Application

WB, IP, FC

Immunogen

A synthesized peptide derived from human Engrailed 1.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IP	1:50
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EN1

Introduction

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal.

Entrez Gene ID

Human	2019
Mouse	13798
Rat	685360

UniProt ID

Human	Q05925
Mouse	P09065
Rat	A0A0G2JT82

Alternative Names

En-1; en-3; Gg-En-1

Biological Process

Adult locomotory behavior Source: Ensembl
Anatomical structure morphogenesis Source: ProtInc
Cerebellum development Source: Ensembl
Dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Dorsal/ventral pattern formation Source: Ensembl
Drinking behavior Source: Ensembl
Embryonic brain development Source: Ensembl
Embryonic forelimb morphogenesis Source: Ensembl
Midbrain development Source: Ensembl
Midbrain-hindbrain boundary development Source: Ensembl
Motor learning Source: Ensembl
Multicellular organism growth Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Neuron development Source: Ensembl
Neuron differentiation Source: GO_Central
Pigmentation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Proximal/distal pattern formation Source: Ensembl
Rregulation of transcription by RNA polymerase II Source: GO_Central
Response to cocaine Source: Ensembl
Skeletal system development Source: ProtInc
Social behavior Source: Ensembl

Cellular Location

Nucleus

Yang, L., Li, X., Luo, Y., Yang, T., Wang, H., Shi, L., ... & Xie, W. (2021). Weighted gene co‑expression network analysis of the association between upregulated AMD1, EN1 and VGLL1 and the progression and poor prognosis of breast cancer. Experimental and Therapeutic Medicine, 22(3), 1-13.

Baba, S., Akashi, T., Kayamori, K., Ohuchi, T., Ogawa, I., Kubota, N., ... & Ikeda, T. (2021). Homeobox transcription factor engrailed homeobox 1 is a possible diagnostic marker for adenoid cystic carcinoma and polymorphous adenocarcinoma. Pathology International, 71(2), 113-123.

Györfi, A. H., Matei, A. E., Fuchs, M., Liang, C., Rigau, A. R., Hong, X., ... & Distler, J. H. (2021). Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation. Journal of Experimental Medicine, 218(9), e20201916.

Miura, K., Akashi, T., Namiki, T., Hishima, T., Bae, Y., Sakurai, U., ... & Eishi, Y. (2020). Engrailed homeobox 1 and cytokeratin 19 are independent diagnostic markers of eccrine porocarcinoma and distinguish it from squamous cell carcinoma. American Journal of Clinical Pathology, 154(4), 499-509.

Zhu, J., & Zhang, Y. Q. (2019). Engrailed 1 overexpression as a potential prognostic marker in Lower Grade Glioma. PeerJ, 7, e7414.

Gandhi, N. S., Blancafort, P., & Mancera, R. L. (2018). Atomistic molecular dynamics simulations of bioactive engrailed 1 interference peptides (EN1-iPeps). Oncotarget, 9(32), 22383.

Miura, K., Akashi, T., Ando, N., Ayabe, S., Kayamori, K., Namiki, T., & Eishi, Y. (2018). Homeobox transcriptional factor engrailed homeobox 1 is expressed specifically in normal and neoplastic sweat gland cells. Histopathology, 72(7), 1199-1208.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-EN1 Recombinant Antibody (1F5) (CAT#: CBMAB-A2599-LY)

Mouse Anti-EN1 Recombinant Antibody (3H2) (CAT#: CBMAB-1142-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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