Mouse Anti-ERAS Recombinant Antibody (CBFYE-1089) (V2LY-0825-LY587)

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Basic Information

Host Animal
Mouse
Clone
CBFYE-1089
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 186-227 mapping at the C-terminus of mouse E-Ras.
Host Species
Mouse
Specificity
Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ES Cell Expressed Ras
Entrez Gene ID
UniProt ID
Research Area
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity).
Biological Process
Ras protein signal transduction Source: GO_Central
Cellular Location
Cell membrane

Pudewell, S., Lissy, J., Nakhaeizadeh, H., Taha, M. S., Akbarzadeh, M., Rezaei Adariani, S., ... & Ahmadian, M. R. (2022). Physical interaction between embryonic stem cell-expressed Ras (ERas) and Arginase-1 in quiescent hepatic stellate cells. Cells, 11(3), 508.

Suarez-Cabrera, C., Ojeda-Perez, I., Sanchez-Baltasar, R., Page, A., Bravo, A., Navarro, M., & Ramirez, A. (2021). ERAS, a Member of the Ras Superfamily, Acts as an Oncoprotein in the Mammary Gland. Cancers, 13(21), 5588.

Tian, H., Wang, W., Meng, X., Wang, M., Tan, J., Jia, W., ... & Zhou, Q. (2020). ERas enhances resistance to cisplatin-induced apoptosis by suppressing autophagy in gastric cancer cell. Frontiers in cell and developmental biology, 7, 375.

Liu, Y., Qin, P., Wu, R., Du, L., & Li, F. (2020). ERas regulates cell proliferation and epithelial–mesenchymal transition by affecting Erk/Akt signaling pathway in pancreatic cancer. Human cell, 33(4), 1186-1196.

Pan, J., & Zhao, L. X. (2020). Epithelial-mesenchymal transition suppresses ERas to activate autophagy in retinal pigment epithelial cells in proliferative vitreoretinopathy. Eur Rev Med Pharmacol Sci, 24(9), 4627-4634.

Ikink, G. J., Boer, M., Bakker, E. R., Vendel-Zwaagstra, A., Klijn, C., Ten Hoeve, J., ... & Hilkens, J. (2018). Insertional mutagenesis in a HER2-positive breast cancer model reveals ERAS as a driver of cancer and therapy resistance. Oncogene, 37(12), 1594-1609.

Suárez-Cabrera, C., de la Peña, B., González, L. L., Page, A., Martínez-Fernández, M., Casanova, M. L., ... & Navarro, M. (2018). The Ras-related gene ERAS is involved in human and murine breast cancer. Scientific reports, 8(1), 1-17.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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