Mouse Anti-ERP44 Recombinant Antibody (CBFYE-1207) (V2LY-0825-LY741)

Name: Mouse Anti-ERP44 Recombinant Antibody (CBFYE-1207)
SKU: V2LY-0825-LY741
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1207

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 107-406 mapping at the C-terminus of human TXNDC4.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Endoplasmic Reticulum Protein 44

Entrez Gene ID

Human	23071
Mouse	76299
Rat	298066
Monkey	100423317

UniProt ID

Human	Q9BS26
Mouse	Q9D1Q6
Rat	Q5VLR5
Monkey	A0A0D9RFC8

Research Area

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum.

Biological Process

Cell redox homeostasis Source: UniProtKB
Glycoprotein metabolic process Source: UniProtKB
Protein folding Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB

Cellular Location

Endoplasmic reticulum lumen

Yang, Y., Qiu, X., & Wang, F. (2021). Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia. Bioengineered, 12(2), 9561-9574.

Xia, T., Tian, H., Zhang, K., Zhang, S., Chen, W., Shi, S., & You, Y. (2021). Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma. BMC cancer, 21(1), 1-13.

Tempio, T., & Anelli, T. (2020). The pivotal role of ERp44 in patrolling protein secretion. Journal of Cell Science, 133(21), jcs240366.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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