Rabbit Anti-ERp44 Recombinant Antibody (CBFYE-1208) (V2LY-0825-LY742)

Name: Rabbit Anti-ERp44 Recombinant Antibody (CBFYE-1208)
SKU: V2LY-0825-LY742
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ERp44 Recombinant Antibody (CBFYE-1208) (V2LY-0825-LY742) in WB

Cells and media collected from HeLa S3 cells expressing the xDEL variants (mScarlet-xDELsec) indicated in the figure were Western blotted for resident ER chaperones and KDELR. ...View More

Gerondopoulos, A., Bräuer, P., Sobajima, T., Wu, Z., Parker, J. L., Biggin, P. C., ... & Newstead, S. (2021). A signal capture and proofreading mechanism for the KDEL-receptor explains selectivity and dynamic range in ER retrieval. Elife, 10, e68380.

Rabbit Anti-ERp44 Recombinant Antibody (CBFYE-1208) (V2LY-0825-LY742) in WB

A, C Western blot was used to detect the level of ERP44 protein; B immunoprecipitation was used to detect the binding relationship between IP3R1 and ERP44; D Rhod2 AM fluorescent labeling was used to observe the changes of intracellular Ca2+ level. Compared to the blank group, *p < 0.05, ***p < 0.001; for pairwise comparison, ##p < 0.01, ###p < 0.001. All experiments were performed three times independently. Data were analyzed using one-way ANOVA and Tukey's multiple comparison test. ...View More

Mo, G., Liu, X., Zhong, Y., Mo, J., Li, Z., Li, D., ... & Liu, Y. (2021). IP3R1 regulates Ca2+ transport and pyroptosis through the NLRP3/Caspase-1 pathway in myocardial ischemia/reperfusion injury. Cell death discovery, 7(1), 31.

Rabbit Anti-ERp44 Recombinant Antibody (CBFYE-1208) (V2LY-0825-LY742) in WB

Protein expression of ER-localized oxidoreductases in HDFs (P30) treated without or with 200 μm metformin for 24 hr. ...View More

Fang, J., Yang, J., Wu, X., Zhang, G., Li, T., Wang, X. E., ... & Wang, L. (2018). Metformin alleviates human cellular aging by upregulating the endoplasmic reticulum glutathione peroxidase 7. Aging cell, 17(4), e12765.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYE-1208

Application

WB, IHC

Immunogen

Synthetic peptide corresponding to the sequence of human ERp44.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IHC	1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, BSA, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Endoplasmic Reticulum Protein 44

Entrez Gene ID

Human	23071
Mouse	76299
Rat	298066
Monkey	100423317

UniProt ID

Human	Q9BS26
Mouse	Q9D1Q6
Rat	Q5VLR5
Monkey	A0A0D9RFC8

Research Area

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum.

Biological Process

Cell redox homeostasis Source: UniProtKB
Glycoprotein metabolic process Source: UniProtKB
Protein folding Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB

Cellular Location

Endoplasmic reticulum lumen

Yang, Y., Qiu, X., & Wang, F. (2021). Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia. Bioengineered, 12(2), 9561-9574.

Xia, T., Tian, H., Zhang, K., Zhang, S., Chen, W., Shi, S., & You, Y. (2021). Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma. BMC cancer, 21(1), 1-13.

Tempio, T., & Anelli, T. (2020). The pivotal role of ERp44 in patrolling protein secretion. Journal of Cell Science, 133(21), jcs240366.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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