Mouse Anti-EVE Monoclonal Antibody (2B8) (CBMAB-0127-CN)

Name: Mouse Anti-EVE Monoclonal Antibody (2B8)
SKU: CBMAB-0127-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EVE Monoclonal Antibody (2B8) (CBMAB-0127-CN) in IF

Col is expressed in discrete subsets of interneurons.

Stage 15 embryos stained with Col and different interneuron markers. (A-A″) Col is expressed by the dAp (dorsal Apterous) interneuron (ApGal4+) and by the dCID interneuron (indicated by arrows). (B-B″) In the dorsal region of the VNC Col is only found expressed in one Dachshund (DacGal4) expressing interneuron that we have named dCID (dorsal Col, Islet, Dachshund) neuron (see also Figure 2). Note that the dCID is found at the vicinity of dAp. (C-C″) In the intermediate region of the VNC Col expression is found in 2 laterally located Dac+ interneurons indicated by the arrows. (D-D″) Overlap between Col and Eve is found in 4/5 EL (Eve Lateral) Eve+ interneurons. (E-E″) In the dorsal region of the VNC Col is not expressed with Eagle (EgGal4). Nevertheless note that the dAp and the dCID interneurons are located in a region where two Eg+ neurons are found. Note that here only two consecutive segments are shown. (F-F″) In the intermediate region of the VNC Col is not found expressed in the 3 Eg expressing serotoninergic neurons (EW1-3) but it is expressed in 3/4 Eg+ laterally located interneurons that belong the EL (Eve+) cluster. (G-G″) Col and Engrailed (En) expression only overlaps in 2 interneurons that are located in the lateral and intermediate region of the VNC. ...View More

Demilly, A., Simionato, E., Ohayon, D., Kerner, P., Garces, A., & Vervoort, M. (2011). Coe genes are expressed in differentiating neurons in the central nervous system of protostomes. PloS one, 6(6), e21213.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2B8

Application

IF, IHC, WB

Immunogen

Recombinant even-skipped protein.

Host Species

Mouse

Specificity

Common fruit fly

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	20-50 ng/ml
IF(ICC)	0.2-0.5 μg/ml
IHC	0.2-0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Even Skipped

Introduction

Even skipped (Eve) is a homeobox-containing transcriptional repressor that acts in concert with co-repressors such as gro and Gug. Eve represses pair-rule and segment polarity genes, contributing to anterior/posterior axis specification and mesoderm and central nervous system development. The protein may play a role in determining neuronal identity, and may be directly involved in specifying identity of individual neurons.

Entrez Gene ID

36039

UniProt ID

P06602

Alternative Names

10.5; 10.9; 14.10; 20.35; CG2328; dm-eve; Dmel\CG2328; E(eve); Eve; EVE; eve2; even; F; l(2)46Ce; l(2)46CFg; l(2)46CFh; l(2)46CFj; l(2)46CFp; l(2)46Cg; V; VI

Research Area

May play a role in determining neuronal identity. May be directly involved in specifying identity of individual neurons. Pair-rule protein required for segmentation; involved in transforming the broad, spatial, aperiodic expression patterns of the gap genes into a system of precise periodic expression patterns of the pair-rule and segmentary polarity genes.

Biological Process

Adult heart development Source: FlyBase
Blastoderm segmentation Source: FlyBase
Cephalic furrow formation Source: FlyBase
Germ-band extension Source: FlyBase
Heart morphogenesis Source: FlyBase
Motor neuron axon guidance Source: FlyBase
Negative regulation of cardioblast cell fate specification Source: FlyBase
Negative regulation of transcription by RNA polymerase II Source: FlyBase
Periodic partitioning by pair rule gene Source: FlyBase
Positive regulation of transcription by RNA polymerase II Source: FlyBase
Posterior head segmentation Source: FlyBase
Regulation of axonogenesis Source: FlyBase
Regulation of myoblast fusion Source: FlyBase
Regulation of transcription by RNA polymerase II Source: GO_Central
Trunk segmentation Source: FlyBase

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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