Mouse Anti-EVE Monoclonal Antibody (3C10) (CBMAB-0175-CN)

Name: Mouse Anti-EVE Monoclonal Antibody (3C10)
SKU: CBMAB-0175-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EVE Monoclonal Antibody (3C10) (CBMAB-0175-CN) in IF

Immunostaining for myc-tagged Opa expression relative to expression of pair-rule genes Runt and Eve in a late NC14 embryo. ...View More

Soluri, I. V., Zumerling, L. M., Payan Parra, O. A., Clark, E. G., & Blythe, S. A. (2020). Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network. Elife, 9, e53916.

Mouse Anti-EVE Monoclonal Antibody (3C10) (CBMAB-0175-CN) in IF

Presynaptic Rabankyrin or CtBP RNAi increases P-Mad in motor neurons. Single confocal sections of NMJ 6/7 (top) and ventral nerve cord (VNC; bottom) co-labeled for anti-P-Mad and anti-HRP (NMJ) or anti-Eve (VNC). ...View More

Kim, N., Kim, S., Nahm, M., Kopke, D., Kim, J., Cho, E., ... & Lee, S. (2019). BMP-dependent synaptic development requires Abi-Abl-Rac signaling of BMP receptor macropinocytosis. Nature communications, 10(1), 684.

Mouse Anti-EVE Monoclonal Antibody (3C10) (CBMAB-0175-CN) in IF

Bcd (green) and Eve (red) proteins in embryos from otherwise wild type females that bear a single copy of a bcd transgene with a wild type (+) (left) or mutant (mut) NRE (right). ...View More

Wharton, T. H., Nomie, K. J., & Wharton, R. P. (2018). No significant regulation of bicoid mRNA by Pumilio or Nanos in the early Drosophila embryo. Plos one, 13(3), e0194865.

Mouse Anti-EVE Monoclonal Antibody (3C10) (CBMAB-0175-CN) in IHC

CF2 is involved in determining the number of nuclei in Dorsal Acute 1 muscle (DA1) from segments A3 and A4.

Anti-eve (magenta) and Anti Mef2 (blue) staining of DA1 muscles from yw (A-A"), homozygous Df2γ27 (B-B"), homozygous Mef2>CF2i (C-C"), heterozygous Mef2>CF2 (D-D") and homozygous Mef2>Mef2 stage 17 embryos. Left panels show eve stained nuclei, middle panels show Mef2 staining nuclei and right panels merge. Note that pericardial cells (*) do not express Mef2. It can be observed that eve expressing muscular nuclei number increase or decrease its number according to CF2 expression levels. In panels D, + stands for TM3Ser Act5C-GFP. Segments A3 and A4 are delimited by dotted lines. Pictures were collected using the same settings and images were equally processed. They correspond to maximum projections collected with maximum intensity. ...View More

Arredondo, J. J., Vivar, J., Laine-Menéndez, S., Martínez-Morentin, L., & Cervera, M. (2017). CF2 transcription factor is involved in the regulation of Mef2 RNA levels, nuclei number and muscle fiber size. PLoS One, 12(6), e0179194.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3C10

Application

IHC

Immunogen

C-terminal 2/3 of even-skipped.

Host Species

Mouse

Specificity

Common fruit fly

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	0.2-0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Even Skipped

Introduction

Even skipped (Eve) is a homeobox-containing transcriptional repressor that acts in concert with co-repressors such as gro and Gug. Eve represses pair-rule and segment polarity genes, contributing to anterior/posterior axis specification and mesoderm and central nervous system development. The protein may play a role in determining neuronal identity, and may be directly involved in specifying identity of individual neurons.

Entrez Gene ID

36039

UniProt ID

P06602

Alternative Names

10.5; 10.9; 14.10; 20.35; CG2328; dm-eve; Dmel\CG2328; E(eve); Eve; EVE; eve2; even; F; l(2)46Ce; l(2)46CFg; l(2)46CFh; l(2)46CFj; l(2)46CFp; l(2)46Cg; V; VI

Research Area

May play a role in determining neuronal identity. May be directly involved in specifying identity of individual neurons. Pair-rule protein required for segmentation; involved in transforming the broad, spatial, aperiodic expression patterns of the gap genes into a system of precise periodic expression patterns of the pair-rule and segmentary polarity genes.

Biological Process

Adult heart development Source: FlyBase
Blastoderm segmentation Source: FlyBase
Cephalic furrow formation Source: FlyBase
Germ-band extension Source: FlyBase
Heart morphogenesis Source: FlyBase
Motor neuron axon guidance Source: FlyBase
Negative regulation of cardioblast cell fate specification Source: FlyBase
Negative regulation of transcription by RNA polymerase II Source: FlyBase
Periodic partitioning by pair rule gene Source: FlyBase
Positive regulation of transcription by RNA polymerase II Source: FlyBase
Posterior head segmentation Source: FlyBase
Regulation of axonogenesis Source: FlyBase
Regulation of myoblast fusion Source: FlyBase
Regulation of transcription by RNA polymerase II Source: GO_Central
Trunk segmentation Source: FlyBase

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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