Mouse Anti-EVL Recombinant Antibody (1D6) (CBMAB-A2740-LY)

Name: Mouse Anti-EVL Recombinant Antibody (1D6)
SKU: CBMAB-A2740-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D6

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 326-390 mapping near the C-terminus of human EVL.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Enah/Vasp-like

Entrez Gene ID

51466

UniProt ID

Q9UI08

Alternative Names

RNB6

Research Area

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.

Biological Process

Actin filament organization Source: ProtInc
Actin nucleation Source: InterPro
Actin polymerization or depolymerization Source: UniProtKB
Animal organ morphogenesis Source: UniProtKB
Cell surface receptor signaling pathway Source: UniProtKB
Negative regulation of epithelial cell migration Source: UniProtKB
Negative regulation of ruffle assembly Source: UniProtKB
Nervous system development Source: UniProtKB
Positive regulation of actin filament polymerization Source: GO_Central
Positive regulation of stress fiber assembly Source: UniProtKB
Protein homotetramerization Source: InterPro

Cellular Location

Cytoskeleton; Stress fiber; Lamellipodium. Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes.

PTM

Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.By similarity

Wang, X., Xia, X., Xu, E., Yang, Z., Shen, X., Du, S., ... & Guan, W. (2021). Estrogen Receptor Beta Prevents Signet Ring Cell Gastric Carcinoma Progression in Young Patients by Inhibiting Pseudopodia Formation via the mTOR–Arpc1b/EVL Signaling Pathway. Frontiers in Cell and Developmental Biology, 8, 592919.

Mascarenhas, J. B., Novak, S. M., Mayfield, R. M., Zarate, K. A., Mendoza, T. R., Gregorio, C. C., & Garcia, J. G. N. (2019). The Enah/VASP Like Protein Is Involved in Vascular Remodeling at the Lamellipodia in Endothelial Cells. In C60. VASCULAR BIOLOGY IN LUNG DISEASE (pp. A5295-A5295). American Thoracic Society.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-EVL Recombinant Antibody (CBFYE-0277) (CAT#: CBMAB-E0556-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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