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Mouse Anti-EXOSC4 Recombinant Antibody (CBFYE-0287) (V2LY-0825-LY961)

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Published Data
V2LY-0825-LY961-1
Mouse Anti-EXOSC4 Recombinant Antibody (CBFYE-0287) (V2LY-0825-LY961) in Immunoblot
Subcellular distribution of Flag-USP36, EXOSC10 and EXOSC4 proteins. HeLa cells were fractionated into cytoplasmic (Cyto), nucleoplasmic (Np) and nucleolar fractions (No) followed by IB. Tubulin, SP1 and FBL were used as cytoplasmic, nucleoplasmic and nucleolar markers, respectively. ...View More
Chen, Y., Li, Y., Dai, R. S., Savage, J. C., Shinde, U., Klimek, J., ... & Dai, M. S. (2023). The ubiquitin-specific protease USP36 SUMOylates EXOSC10 and promotes the nucleolar RNA exosome function in rRNA processing. Nucleic acids research, 51(8), 3934-3949.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYE-0287
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 2-243 of human EXOSC4.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
exosome component 4
Entrez Gene ID
54512
UniProt ID
Q9NPD3
Research Area
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs.
Biological Process
Defense response to virus Source: MGI
DNA deamination Source: UniProtKB
Histone mRNA catabolic process Source: UniProtKB
Maturation of 5.8S rRNA Source: UniProtKB
Nuclear mRNA surveillance Source: UniProtKB
Nuclear-transcribed mRNA catabolic process Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: GO_Central
Polyadenylation-dependent snoRNA 3'-end processing Source: GO_Central
Positive regulation of cell growth Source: UniProtKB
rRNA catabolic process Source: GO_Central
rRNA processing Source: UniProtKB
U4 snRNA 3'-end processing Source: GO_Central
Cellular Location
Nucleolus; Nucleus; Cytoplasm

Taniue, K., Tanu, T., Shimoura, Y., Mitsutomi, S., Han, H., Kakisaka, R., ... & Akimitsu, N. (2022). RNA Exosome Component EXOSC4 Amplified in Multiple Cancer Types Is Required for the Cancer Cell Survival. International journal of molecular sciences, 23(1), 496.

Wang, Y. X., Li, Y. Z., Zhu, H. F., Zhang, Z. Y., Qian, X. L., & He, G. Y. (2020). STX2 drives colorectal cancer proliferation via upregulation of EXOSC4. Life Sciences, 263, 118597.

Bera, A., Karaian, J., Subramanian, M., Eklund, M., Russ, E., Pollard, H. B., ... & Srivastava, M. (2020). EXOSC4, a novel gene at chromosome 8q24 loci is linked with breast cancer progression and is a prognostic marker for breast cancer survival. Cancer Research, 80(16_Supplement), 4322-4322.

Pan, Y., Tong, J. H., Kang, W., Lung, R. W., Chak, W. P., Chung, L. Y., ... & To, K. F. (2018). EXOSC4 functions as a potential oncogene in development and progression of colorectal cancer. Molecular Carcinogenesis, 57(12), 1780-1791.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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