Rabbit Anti-EZH1 Recombinant Antibody (5D) (CBMAB-CP0656-LY)

Name: Rabbit Anti-EZH1 Recombinant Antibody (5D)
SKU: CBMAB-CP0656-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of human EZH1 protein.

Host Species

Rabbit

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Enhancer Of Zeste 1 Polycomb Repressive Complex 2 Subunit

Introduction

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

Entrez Gene ID

Human	2145
Mouse	14055
Rat	303547

UniProt ID

Human	Q92800
Mouse	P70351
Rat	F1LZH3

Alternative Names

Enhancer Of Zeste 1 Polycomb Repressive Complex 2 Subunit; Enhancer Of Zeste Homolog 1; EC 2.1.1.43; ENX-2; Enhancer Of Zeste (Drosophila) Homolog 1; Enhancer Of Zeste Homolog 1 (Drosophila); Histone-Lysine N-Methyltransferase EZH1; KIAA0388; KMT6B;

Research Area

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Biological Process

Anatomical structure morphogenesis Source: ProtInc
Chromatin remodeling Source: ARUK-UCL
Chromatin silencing at telomere Source: ARUK-UCL
Heterochromatin assembly Source: ARUK-UCL
Hippocampus development Source: Ensembl
Histone H3-K27 methylation Source: InterPro
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of transcription by RNA polymerase II Source: Ensembl

Cellular Location

Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.

Jing, R., Scarfo, I., Najia, M. A., da Rocha, E. L., Han, A., Sanborn, M., ... & Daley, G. Q. (2022). EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity. Cell Stem Cell, 29(8), 1181-1196.

Grau, D., Zhang, Y., Lee, C. H., Valencia-Sánchez, M., Zhang, J., Wang, M., ... & Armache, K. J. (2021). Structures of monomeric and dimeric PRC2: EZH1 reveal flexible modules involved in chromatin compaction. Nature communications, 12(1), 1-12.

Kusakabe, Y., Chiba, T., Oshima, M., Koide, S., Rizq, O., Aoyama, K., ... & Kato, N. (2021). EZH1/2 inhibition augments the anti-tumor effects of sorafenib in hepatocellular carcinoma. Scientific reports, 11(1), 1-14.

El Said, N. H., Della Valle, F., Liu, P., Paytuví-Gallart, A., Adroub, S., Gimenez, J., & Orlando, V. (2021). Malat-1-PRC2-EZH1 interaction supports adaptive oxidative stress dependent epigenome remodeling in skeletal myotubes. Cell death & disease, 12(10), 1-10.

Kagiyama, Y., Fujita, S., Shima, Y., Yamagata, K., Katsumoto, T., Nakagawa, M., ... & Kitabayashi, I. (2021). CDKN1C‐mediated growth inhibition by an EZH1/2 dual inhibitor overcomes resistance of mantle cell lymphoma to ibrutinib. Cancer Science, 112(6), 2314-2324.

Lau-Corona, D., Bae, W. K., Hennighausen, L., & Waxman, D. J. (2020). Sex-biased genetic programs in liver metabolism and liver fibrosis are controlled by EZH1 and EZH2. PLoS genetics, 16(5), e1008796.

Wassef, M., Luscan, A., Aflaki, S., Zielinski, D., Jansen, P. W., Baymaz, H. I., ... & Margueron, R. (2019). EZH1/2 function mostly within canonical PRC2 and exhibit proliferation-dependent redundancy that shapes mutational signatures in cancer. Proceedings of the National Academy of Sciences, 116(13), 6075-6080.

Jung, C. K., Kim, Y., Jeon, S., Jo, K., Lee, S., & Bae, J. S. (2018). Clinical utility of EZH1 mutations in the diagnosis of follicular-patterned thyroid tumors. Human Pathology, 81, 9-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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