Human Recombinant EZH1 protein, GST Tag (V2LY-0526-LY3972)

Name: Human Recombinant EZH1 protein, GST Tag
SKU: V2LY-0526-LY3972
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant EZH1 protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 130 kDa.

Molecule Mass

130 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Enhancer Of Zeste 1 Polycomb Repressive Complex 2 Subunit

Research Area

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Biological Process

Anatomical structure morphogenesis Source: ProtInc
Chromatin remodeling Source: ARUK-UCL
Chromatin silencing at telomere Source: ARUK-UCL
Heterochromatin assembly Source: ARUK-UCL
Hippocampus development Source: Ensembl
Histone H3-K27 methylation Source: InterPro
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of transcription by RNA polymerase II Source: Ensembl

Cellular Location

Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.

Jing, R., Scarfo, I., Najia, M. A., da Rocha, E. L., Han, A., Sanborn, M., ... & Daley, G. Q. (2022). EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity. Cell Stem Cell, 29(8), 1181-1196.

Grau, D., Zhang, Y., Lee, C. H., Valencia-Sánchez, M., Zhang, J., Wang, M., ... & Armache, K. J. (2021). Structures of monomeric and dimeric PRC2: EZH1 reveal flexible modules involved in chromatin compaction. Nature communications, 12(1), 1-12.

Kusakabe, Y., Chiba, T., Oshima, M., Koide, S., Rizq, O., Aoyama, K., ... & Kato, N. (2021). EZH1/2 inhibition augments the anti-tumor effects of sorafenib in hepatocellular carcinoma. Scientific reports, 11(1), 1-14.

El Said, N. H., Della Valle, F., Liu, P., Paytuví-Gallart, A., Adroub, S., Gimenez, J., & Orlando, V. (2021). Malat-1-PRC2-EZH1 interaction supports adaptive oxidative stress dependent epigenome remodeling in skeletal myotubes. Cell death & disease, 12(10), 1-10.

Kagiyama, Y., Fujita, S., Shima, Y., Yamagata, K., Katsumoto, T., Nakagawa, M., ... & Kitabayashi, I. (2021). CDKN1C‐mediated growth inhibition by an EZH1/2 dual inhibitor overcomes resistance of mantle cell lymphoma to ibrutinib. Cancer Science, 112(6), 2314-2324.

Lau-Corona, D., Bae, W. K., Hennighausen, L., & Waxman, D. J. (2020). Sex-biased genetic programs in liver metabolism and liver fibrosis are controlled by EZH1 and EZH2. PLoS genetics, 16(5), e1008796.

Wassef, M., Luscan, A., Aflaki, S., Zielinski, D., Jansen, P. W., Baymaz, H. I., ... & Margueron, R. (2019). EZH1/2 function mostly within canonical PRC2 and exhibit proliferation-dependent redundancy that shapes mutational signatures in cancer. Proceedings of the National Academy of Sciences, 116(13), 6075-6080.

Jung, C. K., Kim, Y., Jeon, S., Jo, K., Lee, S., & Bae, J. S. (2018). Clinical utility of EZH1 mutations in the diagnosis of follicular-patterned thyroid tumors. Human Pathology, 81, 9-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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