Mouse Anti-FAAH Recombinant Antibody (5F9) (CBMAB-A2790-LY)

Name: Mouse Anti-FAAH Recombinant Antibody (5F9)
SKU: CBMAB-A2790-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FAAH Recombinant Antibody (5F9) (CBMAB-A2790-LY) in Immunoblot

Basal differences in AEA and FAAH levels in the brain of WKY rats.

The level of eCB, AEA was found to be significantly lower in hippocampus of WKY rats compared to WIS rats (31%, p<0.01; A). Conversely, the level of FAAH enzyme was significantly higher in frontal cortex (40%, p<0.05) and hippocampus (40%, p<0.05; B) of WKY rats. A representative immunoblot for hippocampus is provided in the upper panel (B). The qPCR analysis also indicated higher levels of mFAAH in hippocampus of WKY rats (24%, p<0.05; C). The qPCR data on FAAH, normalized to β-Actin (internal standard) is presented as the fold change relative to the control value of 1.0. The FAAH activity was slightly higher in frontal cortex (15%, p<0.05) and hippocampus (17%, p<0.05) of WKY rats compared to WIS rats (D). Hippo; Hippocampus. ...View More

Vinod, K. Y., Xie, S., Psychoyos, D., Hungund, B. L., Cooper, T. B., & Tejani-Butt, S. M. (2012). Dysfunction in fatty acid amide hydrolase is associated with depressive-like behavior in Wistar Kyoto rats. PloS one, 7(5), e36743.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5F9

Application

WB, ELISA

Immunogen

FAAH (aa 480-579) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fatty Acid Amide Hydrolase

Introduction

This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq]

Entrez Gene ID

Human	2166
Mouse	14073
Rat	100911581

UniProt ID

Human	O00519
Mouse	O08914
Rat	P97612

Alternative Names

FAAH-1; MGC102823; MGC138146

Research Area

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:9122178, PubMed:17015445, PubMed:19926788).

Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed:9122178, PubMed:17015445).

It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed:21049984).

FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

Biological Process

Arachidonic acid metabolic process Source: Reactome
Fatty acid catabolic process Source: UniProtKB
Monoacylglycerol catabolic process Source: UniProtKB

Cellular Location

Cytoskeleton; Endomembrane system. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Genovese, T., Duranti, A., D’Amico, R., Fusco, R., Impellizzeri, D., Peritore, A. F., ... & Cordaro, M. (2022). Fatty Acid Amide Hydrolase (FAAH) Inhibition Plays a Key Role in Counteracting Acute Lung Injury. International Journal of Molecular Sciences, 23(5), 2781.

Grieco, M., De Caris, M. G., Maggi, E., Armeli, F., Coccurello, R., Bisogno, T., ... & Businaro, R. (2021). Fatty acid amide hydrolase (FAAH) inhibition modulates amyloid-beta-induced microglia polarization. International Journal of Molecular Sciences, 22(14), 7711.

Rafiei, D., & Kolla, N. J. (2021). Elevated brain fatty acid amide hydrolase induces depressive-like phenotypes in rodent models: A review. International Journal of Molecular Sciences, 22(3), 1047.

Matheson, J., Zhou, X. M. M., Bourgault, Z., & Le Foll, B. (2021). Potential of fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MAGL), and diacylglycerol lipase (DAGL) enzymes as targets for obesity treatment: A narrative review. Pharmaceuticals, 14(12), 1316.

Schmidt, M. E., Liebowitz, M. R., Stein, M. B., Grunfeld, J., Van Hove, I., Simmons, W. K., ... & Drevets, W. C. (2021). The effects of inhibition of fatty acid amide hydrolase (FAAH) by JNJ-42165279 in social anxiety disorder: a double-blind, randomized, placebo-controlled proof-of-concept study. Neuropsychopharmacology, 46(5), 1004-1010.

Chiurchiù, V., Scipioni, L., Arosio, B., Mari, D., Oddi, S., & Maccarrone, M. (2021). Anti-inflammatory effects of fatty acid amide hydrolase inhibition in monocytes/macrophages from alzheimer’s disease patients. Biomolecules, 11(4), 502.

Tripathi, R. K. P. (2020). A perspective review on fatty acid amide hydrolase (FAAH) inhibitors as potential therapeutic agents. European Journal of Medicinal Chemistry, 188, 111953.

Mayo, L. M., Asratian, A., Lindé, J., Morena, M., Haataja, R., Hammar, V., ... & Heilig, M. (2020). Elevated anandamide, enhanced recall of fear extinction, and attenuated stress responses following inhibition of fatty acid amide hydrolase: a randomized, controlled experimental medicine trial. Biological psychiatry, 87(6), 538-547.

Brunetti, L., Loiodice, F., Piemontese, L., Tortorella, P., & Laghezza, A. (2019). New Approaches to Cancer Therapy: Combining Fatty Acid Amide Hydrolase (FAAH) Inhibition with Peroxisome Proliferator-Activated Receptors (PPARs) Activation: Miniperspective. Journal of Medicinal Chemistry, 62(24), 10995-11003.

Balsevich, G., Sticht, M., Bowles, N. P., Singh, A., Lee, T. T., Li, Z., ... & Hill, M. N. (2018). Role for fatty acid amide hydrolase (FAAH) in the leptin-mediated effects on feeding and energy balance. Proceedings of the National Academy of Sciences, 115(29), 7605-7610.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FAAH Matched Antibody Pair (397) (CAT#: APMAB-397LY)

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Isotype control

For research use only. Not intended for any clinical use.

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