Rabbit Anti-FAAH Recombinant Antibody (
8G3) (V2LY-0725-LY1245)

Name: Rabbit Anti-FAAH Recombinant Antibody (8G3)
SKU: V2LY-0725-LY1245
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-FAAH Recombinant Antibody (8G3) (V2LY-0725-LY1245) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate
All lanes: FAAH1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa

Rabbit Anti-FAAH Recombinant Antibody (8G3) (V2LY-0725-LY1245) in IHC

IHC image of V2LY-0725-LY1245 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

8G3

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human FAAH1.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fatty Acid Amide Hydrolase

Entrez Gene ID

2166

UniProt ID

O00519

Research Area

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:9122178, PubMed:17015445, PubMed:19926788).

Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed:9122178, PubMed:17015445).

It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed:21049984).

FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

Biological Process

Arachidonic acid metabolic process Source: Reactome
Fatty acid catabolic process Source: UniProtKB
Monoacylglycerol catabolic process Source: UniProtKB

Cellular Location

Cytoskeleton; Endomembrane system. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Genovese, T., Duranti, A., D’Amico, R., Fusco, R., Impellizzeri, D., Peritore, A. F., ... & Cordaro, M. (2022). Fatty Acid Amide Hydrolase (FAAH) Inhibition Plays a Key Role in Counteracting Acute Lung Injury. International Journal of Molecular Sciences, 23(5), 2781.

Grieco, M., De Caris, M. G., Maggi, E., Armeli, F., Coccurello, R., Bisogno, T., ... & Businaro, R. (2021). Fatty acid amide hydrolase (FAAH) inhibition modulates amyloid-beta-induced microglia polarization. International Journal of Molecular Sciences, 22(14), 7711.

Rafiei, D., & Kolla, N. J. (2021). Elevated brain fatty acid amide hydrolase induces depressive-like phenotypes in rodent models: A review. International Journal of Molecular Sciences, 22(3), 1047.

Matheson, J., Zhou, X. M. M., Bourgault, Z., & Le Foll, B. (2021). Potential of fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MAGL), and diacylglycerol lipase (DAGL) enzymes as targets for obesity treatment: A narrative review. Pharmaceuticals, 14(12), 1316.

Schmidt, M. E., Liebowitz, M. R., Stein, M. B., Grunfeld, J., Van Hove, I., Simmons, W. K., ... & Drevets, W. C. (2021). The effects of inhibition of fatty acid amide hydrolase (FAAH) by JNJ-42165279 in social anxiety disorder: a double-blind, randomized, placebo-controlled proof-of-concept study. Neuropsychopharmacology, 46(5), 1004-1010.

Chiurchiù, V., Scipioni, L., Arosio, B., Mari, D., Oddi, S., & Maccarrone, M. (2021). Anti-inflammatory effects of fatty acid amide hydrolase inhibition in monocytes/macrophages from alzheimer’s disease patients. Biomolecules, 11(4), 502.

Tripathi, R. K. P. (2020). A perspective review on fatty acid amide hydrolase (FAAH) inhibitors as potential therapeutic agents. European Journal of Medicinal Chemistry, 188, 111953.

Mayo, L. M., Asratian, A., Lindé, J., Morena, M., Haataja, R., Hammar, V., ... & Heilig, M. (2020). Elevated anandamide, enhanced recall of fear extinction, and attenuated stress responses following inhibition of fatty acid amide hydrolase: a randomized, controlled experimental medicine trial. Biological psychiatry, 87(6), 538-547.

Brunetti, L., Loiodice, F., Piemontese, L., Tortorella, P., & Laghezza, A. (2019). New Approaches to Cancer Therapy: Combining Fatty Acid Amide Hydrolase (FAAH) Inhibition with Peroxisome Proliferator-Activated Receptors (PPARs) Activation: Miniperspective. Journal of Medicinal Chemistry, 62(24), 10995-11003.

Balsevich, G., Sticht, M., Bowles, N. P., Singh, A., Lee, T. T., Li, Z., ... & Hill, M. N. (2018). Role for fatty acid amide hydrolase (FAAH) in the leptin-mediated effects on feeding and energy balance. Proceedings of the National Academy of Sciences, 115(29), 7605-7610.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FAAH Matched Antibody Pair (397) (CAT#: APMAB-397LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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