Rabbit Anti-FEZ1 Recombinant Antibody (D9) (CBMAB-CP0695-LY)

Name: Rabbit Anti-FEZ1 Recombinant Antibody (D9)
SKU: CBMAB-CP0695-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

Application

WB, IP

Immunogen

Synthetic peptide of human FEZ1 protein.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES & BSA & Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fasciculation And Elongation Protein Zeta 1

Introduction

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	9638
Mouse	235180
Rat	81730

UniProt ID

Human	Q99689
Mouse	Q8K0X8
Rat	P97577

Alternative Names

Fasciculation And Elongation Protein Zeta 1; Zygin I; Zygin-1; Fasciculation And Elongation Protein Zeta-1; UNC-76;

Research Area

May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.

Biological Process

Axon guidance Source: ProtInc
Cell adhesion Source: ProtInc
Cellular response to growth factor stimulus Source: Ensembl
Establishment of cell polarity Source: Ensembl
Establishment of mitochondrion localization Source: Ensembl
Hippocampus development Source: Ensembl
Mitochondrion morphogenesis Source: Ensembl
Negative regulation of autophagosome assembly Source: GO_Central
Nervous system development Source: ProtInc
Positive regulation of anterograde axonal transport of mitochondrion Source: Ensembl
Positive regulation of neuron differentiation Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl

Cellular Location

Centrosome; Cell membrane. Colocalizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta (By similarity).

PTM

Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.
Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.

Razar, R. B. B. A., Qu, Y., Gunaseelan, S., & Chua, J. J. E. (2022). The importance of fasciculation and elongation protein zeta-1 in neural circuit establishment and neurological disorders. Neural Regeneration Research, 17(6), 1165.

Malikov, V., Meade, N., Simons, L. M., Hultquist, J. F., & Naghavi, M. H. (2022). FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression. Cell reports, 38(7), 110396.

Chua, J. Y., Ng, S. J., Yagensky, O., Wanker, E. E., & Chua, J. J. E. (2021). FEZ1 forms complexes with CRMP1 and DCC to regulate axon and dendrite development. Eneuro, 8(2).

Zhang, Y., Shi, Q., Li, X., & Xia, C. (2020). Fasciculation and Elongation Protein Zeta-1 Expression in Reactive Astrocytes in a Rat Model of Frontal Lobe Injury. Journal of Neuropathology & Experimental Neurology, 79(2), 194-208.

Demir, M., Yilmaz, U., Colak, C., Cigremis, Y. I. L. M. A. Z., Ozyalin, F., Tekedereli, I., & Sandal, S. (2019). Is there a new pathway relationship between melatonin and FEZ1 in experimental rat model of Alzheimer's disease?. Bratislavske Lekarske Listy, 120(1), 70-77.

Teixeira, M. B., Alborghetti, M. R., & Kobarg, J. (2019). Fasciculation and elongation zeta proteins 1 and 2: From structural flexibility to functional diversity. World Journal of Biological Chemistry, 10(2), 28.

Bertini Teixeira, M., Figueira, A. C. M., Furlan, A. S., Aquino, B., Alborghetti, M. R., Paes Leme, A. F., ... & Kobarg, J. (2018). Fasciculation and elongation zeta‐1 protein (FEZ1) interacts with the retinoic acid receptor and participates in transcriptional regulation of the Hoxb4 gene. FEBS Open bio, 8(1), 4-14.

Alborghetti, M. R. (2018). Proteínas da família FEZ (Fasciculation and Elongation protein Zeta) como adaptadoras bivalentes do transporte= aspectos funcionais, estruturais e evolutivos (Doctoral dissertation, Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FEZ1 Matched Antibody Pair (423) (CAT#: APMAB-423LY)

Related Products

FEZ1 Matched Antibody Pair (423) (CAT#: APMAB-423LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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