Mouse Anti-FGF16 Recombinant Antibody (CBXF-3339) (V2LY-0925-LY1465)

Name: Mouse Anti-FGF16 Recombinant Antibody (CBXF-3339)
SKU: V2LY-0925-LY1465
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-3339

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 19-53 of human FGF-16.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Pig, Horse

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fibroblast Growth Factor 16

Entrez Gene ID

8823

UniProt ID

O43320

Research Area

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development.

Biological Process

Animal organ morphogenesis Source: GO_Central
Cell-cell signaling Source: ProtInc
Cell differentiation Source: GO_Central
Fibroblast growth factor receptor signaling pathway Source: GO_Central
Positive regulation of brown fat cell proliferation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of endothelial cell chemotaxis to fibroblast growth factor Source: UniProtKB
Positive regulation of gene expression Source: GO_Central
Positive regulation of protein phosphorylation Source: GO_Central
Regulation of cell migration Source: GO_Central
Response to temperature stimulus Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Secreted

Involvement in disease

Metacarpal 4-5 fusion (MF4):
A rare congenital malformation of the hand characterized by the partial or complete fusion of the fourth and fifth metacarpals. The anomaly occurs as an isolated trait or part of a syndrome.

Huang, K., Liang, J. J., Lin, Y. Q., Zhu, J. J., Ma, J. Q., & Wang, Y. (2020). Molecular characterization of fibroblast growth factor-16 and its role in promoting the differentiation of intramuscular preadipocytes in goat. animal, 14(11), 2351-2362.

Huang, J., Wang, K., Shiflett, L. A., Brotto, L., Bonewald, L. F., Wacker, M. J., ... & Brotto, M. (2019). Fibroblast growth factor 9 (FGF9) inhibits myogenic differentiation of C2C12 and human muscle cells. Cell Cycle, 18(24), 3562-3580.

Duan, Y., Wang, Y., Li, X., Mo, J., Guo, X., Li, C., ... & Ge, R. S. (2019). Fibroblast growth factor 16 stimulates proliferation but blocks differentiation of rat stem Leydig cells during regeneration. Journal of cellular and molecular medicine, 23(4), 2632-2644.

Elsy, M., Rowbotham, A., Lord, H., Isaacs, H. V., & Pownall, M. E. (2019). Xenopus laevis FGF16 activates the expression of genes coding for the transcription factors Sp5 and Sp5l. International Journal of Developmental Biology, 1-10.

Wang, J. (2018). Fibroblast growth factor-16 and acute doxorubicin cardiotoxicity: a target for early protection.

Wang, J., Xiang, B., Dolinsky, V. W., Kardami, E., & Cattini, P. A. (2018). Cardiac Fgf-16 expression supports cardiomyocyte survival and increases resistance to doxorubicin cytotoxicity. DNA and Cell Biology, 37(11), 866-877.

Wang, S., Li, Y., Jiang, C., & Tian, H. (2018). Fibroblast growth factor 9 subfamily and the heart. Applied microbiology and biotechnology, 102(2), 605-613.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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