Human, Cynomolgus Recombinant FGF16 protein (V2LY-0526-LY7701)

Name: Human, Cynomolgus Recombinant FGF16 protein
SKU: V2LY-0526-LY7701
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human, Cynomolgus

Protein Construction

This product is Human, Cynomolgus Recombinant FGF16 protein consist of Amino Acid: 1-207 and predicts a molecular mass of 23.8 kDa.

Molecule Mass

23.8 kDa

Sequence

Amino Acid: 1-207

Species

Human, Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Fibroblast Growth Factor 16

Research Area

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development.

Biological Process

Animal organ morphogenesis Source: GO_Central
Cell-cell signaling Source: ProtInc
Cell differentiation Source: GO_Central
Fibroblast growth factor receptor signaling pathway Source: GO_Central
Positive regulation of brown fat cell proliferation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of endothelial cell chemotaxis to fibroblast growth factor Source: UniProtKB
Positive regulation of gene expression Source: GO_Central
Positive regulation of protein phosphorylation Source: GO_Central
Regulation of cell migration Source: GO_Central
Response to temperature stimulus Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Secreted

Involvement in disease

Metacarpal 4-5 fusion (MF4):
A rare congenital malformation of the hand characterized by the partial or complete fusion of the fourth and fifth metacarpals. The anomaly occurs as an isolated trait or part of a syndrome.

Huang, K., Liang, J. J., Lin, Y. Q., Zhu, J. J., Ma, J. Q., & Wang, Y. (2020). Molecular characterization of fibroblast growth factor-16 and its role in promoting the differentiation of intramuscular preadipocytes in goat. animal, 14(11), 2351-2362.

Huang, J., Wang, K., Shiflett, L. A., Brotto, L., Bonewald, L. F., Wacker, M. J., ... & Brotto, M. (2019). Fibroblast growth factor 9 (FGF9) inhibits myogenic differentiation of C2C12 and human muscle cells. Cell Cycle, 18(24), 3562-3580.

Duan, Y., Wang, Y., Li, X., Mo, J., Guo, X., Li, C., ... & Ge, R. S. (2019). Fibroblast growth factor 16 stimulates proliferation but blocks differentiation of rat stem Leydig cells during regeneration. Journal of cellular and molecular medicine, 23(4), 2632-2644.

Elsy, M., Rowbotham, A., Lord, H., Isaacs, H. V., & Pownall, M. E. (2019). Xenopus laevis FGF16 activates the expression of genes coding for the transcription factors Sp5 and Sp5l. International Journal of Developmental Biology, 1-10.

Wang, J. (2018). Fibroblast growth factor-16 and acute doxorubicin cardiotoxicity: a target for early protection.

Wang, J., Xiang, B., Dolinsky, V. W., Kardami, E., & Cattini, P. A. (2018). Cardiac Fgf-16 expression supports cardiomyocyte survival and increases resistance to doxorubicin cytotoxicity. DNA and Cell Biology, 37(11), 866-877.

Wang, S., Li, Y., Jiang, C., & Tian, H. (2018). Fibroblast growth factor 9 subfamily and the heart. Applied microbiology and biotechnology, 102(2), 605-613.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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