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Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936)

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Published Data
V2LY-0925-LY1936-1
Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936) in IF
Immunofluorescence staining for MyHC after transfected with FHL2 shRNA and sh-control cells. ...View More
Zhu, Y., Li, P., Dan, X., Kang, X., Ma, Y., & Shi, Y. (2022). miR-377 inhibits proliferation and differentiation of bovine skeletal muscle satellite cells by targeting FHL2. Genes, 13(6), 947.
V2LY-0925-LY1936-2
Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936) in WB
Protein levels of FHL2 and p57 in different cell lines. 100 μg of total protein extract were analyzed by immunoblotting for FHL2 and p57 using a mouse monoclonal FHL2- and a rabbit polyclonal p57-specific antibody. CEM, human leukemic; HEL, human erythroleukemic; HL-1, mouse cardiac muscle; WM35, human melanoma; 293, human embryonic kidney; MCF-7, human breast cancer; HRT-18, human colon cancer; U2OS, human osteosarcoma; HeLa, human cervix carcinoma. The unspecific band at 70 kD (∗) after probing for p57 was used to judge equal loading. ...View More
Kullmann, M. K., Pegka, F., Ploner, C., & Hengst, L. (2021). Stimulation of c-Jun/AP-1-activity by the cell cycle inhibitor p57Kip2. Frontiers in Cell and Developmental Biology, 9, 664609.
V2LY-0925-LY1936-3
Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936) in IP
co-immunoprecipitation (IP) of endogenous FHL2andmAbp1fromMTLn3celllysate.FHL2wasimmunoprecipitatedwithanti FHL2 but notwithIgGalone,and co-immunoprecipitatedmAbp1 was probed by Western blot. ...View More
Boateng, L. R., Bennin, D., De Oliveira, S., & Huttenlocher, A. (2016). Mammalian actin-binding protein-1/Hip-55 interacts with FHL2 and negatively regulates cell invasion. Journal of Biological Chemistry, 291(27), 13987-13998.
V2LY-0925-LY1936-4
Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936) in IF
Representative images of RFP-FHL2 in FHL2 shRNA cells labeled with Alexa 488 phalloidin (green) and vinculin immunostaining (blue). RFP-FHL2 localizes to focal adhesions and stress fibers and occasionally in the nucleus similar to endogenous FHL2. Scale bar 20m. ...View More
Boateng, L. R., Bennin, D., De Oliveira, S., & Huttenlocher, A. (2016). Mammalian actin-binding protein-1/Hip-55 interacts with FHL2 and negatively regulates cell invasion. Journal of Biological Chemistry, 291(27), 13987-13998.
V2LY-0925-LY1936-5
Mouse Anti-FHL2 Recombinant Antibody (CBXF-0677) (V2LY-0925-LY1936) in WB
Protein expression of SRF network genes in wild type, mild and severe MHC-CELFΔ lines.
Western blot analysis shows a decrease in expression of the SRF inhibitors HOPX and FHL2 in the mild and severe lines of MHC-CELFΔ mice compared to wild type, accompanied by an increase in the levels of three SRF targets. SRF and GAPDH expression levels are constant. Equivalent loading was further confirmed by Ponceau S staining (data not shown). Representatives of three independent blots with similar results are shown. Irrelevant intervening lanes were excised on the ACTA1 blot. ...View More
Dasgupta, T., Stillwagon, S. J., & Ladd, A. N. (2013). Gene expression analyses implicate an alternative splicing program in regulating contractile gene expression and serum response factor activity in mice. PLoS One, 8(2), e56590.
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Basic Information

Host Animal
Mouse
Clone
CBXF-0677
Application
WB, IP, IF, IHC-P
Immunogen
Recombinant human FHL2 protein.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Fibrinogen Like 2
Entrez Gene ID
Human2274
Mouse14200
Rat63839
UniProt ID
HumanQ14192
MouseO70433
RatO35115
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:28717008).
Biological Process
Atrial cardiac muscle cell development Source: Ensembl
Heart trabecula formation Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Osteoblast differentiation Source: Ensembl
Response to hormone Source: BHF-UCL
Ventricular cardiac muscle cell development Source: Ensembl
Cellular Location
Cytoplasm; Nucleus; Z line

Algaber, A., Madhi, R., Hawez, A., Rönnow, C. F., & Rahman, M. (2021). Targeting FHL2‑E‑cadherin axis by miR‑340‑5p attenuates colon cancer cell migration and invasion. Oncology Letters, 22(2), 1-11.

Clemente-Olivo, M. P., Habibe, J. J., Vos, M., Ottenhoff, R., Jongejan, A., Herrema, H., ... & de Vries, C. J. (2021). Four-and-a-half LIM domain protein 2 (FHL2) deficiency protects mice from diet-induced obesity and high FHL2 expression marks human obesity. Metabolism, 121, 154815.

Habibe, J. J., Clemente-Olivo, M. P., & de Vries, C. J. (2021). How (Epi) Genetic Regulation of the LIM-Domain Protein FHL2 Impacts Multifactorial Disease. Cells, 10(10), 2611.

Sommer, J., & Hellerbrand, C. (2020). Expression and Function of Four-and-a-Half LIM-domain protein 2 (FHL2) in Hepatocellular Carcinoma. Zeitschrift für Gastroenterologie, 58(01), 4-18.

Wang, C., Lv, X., He, C., Davis, J. S., Wang, C., & Hua, G. (2020). Four and a half lim domains 2 (FHL2) contribute to the epithelial ovarian cancer carcinogenesis. International journal of molecular sciences, 21(20), 7751.

Sommer, J., Dorn, C., Gäbele, E., Bataille, F., Freese, K., Seitz, T., ... & Hellerbrand, C. (2020). Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury. Cells, 9(1), 248.

Zhang, L., Li, R., Chen, L., Xing, Z., Song, Y., Nie, X., ... & Tian, S. (2020). Expression, location and biological effects of four and a half LIM domain protein 2 (FHL2) on granulosa cells in ovine. Reproduction in Domestic Animals, 55(6), 737-746.

Sommer, J., Dorn, C., Weiskirchen, R., & Hellerbrand, C. (2020). Expression and Function of Four-and-a-Half LIM-domain protein 2 (FHL2) in Hepatic Fibrosis. Zeitschrift für Gastroenterologie, 58(01), 1-7.

Nishikawa, M., Sato, K., Nakano, S., Yamakawa, H., Nagase, T., & Ueda, H. (2019). Specific activation of PLEKHG2-induced serum response element-dependent gene transcription by four-and-a-half LIM domains (FHL) 1, but not FHL2 or FHL3. Small GTPases, 10(5), 361-366.

Liang, Y., Bradford, W. H., Zhang, J., & Sheikh, F. (2018). Four and a half LIM domain protein signaling and cardiomyopathy. Biophysical reviews, 10(4), 1073-1085.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FHL2 Matched Antibody Pair (443) (CAT#: APMAB-443LY)

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FHL2 Matched Antibody Pair (443) (CAT#: APMAB-443LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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