Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089)

Name: Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973)
SKU: V2LY-0925-LY2089
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089) in WB

Relative expression of FKBP51 protein in U937 cells and THP-1 cells, which was confirmed by western blot analysis. ...View More

Sun, H., Liu, X., Wang, L., Cui, B., Mu, W., Xia, Y., ... & Zhao, Y. (2022). Dexamethasone Sensitizes Acute Monocytic Leukemia Cells to Ara-C by Upregulating FKBP51. Frontiers in Oncology, 12, 888695.

Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089) in WB

(A-D) The relative protein and mRNA quantities of FKBP51 in THP-1 and U937 cells conditioned with dexamethasone at a series of concentration relative to that in control cells. Dexamethasone upregulated FKBP51 expression in a dose-dependent manner. ...View More

Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089) in Immunoblot

LNCaP-EnzR cells were treated after 96 h, AR, TMPRSS2, FKBP5 and IKBKE expression was determined by immunoblotting ...View More

Bainbridge, A., Walker, S., Smith, J., Patterson, K., Dutt, A., Ng, Y. M., ... & Coffey, K. (2020). IKBKE activity enhances AR levels in advanced prostate cancer via modulation of the Hippo pathway. Nucleic Acids Research, 48(10), 5366-5382.

Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089) in WB

Cochaperone protein FKBP51 expression normalized against GAPDH. Panels display cortex homogenates separated into nuclear localizations (a) and cytosolic localizations (b) with their respective immunoblots, (c) and (d), below each graph. *p < .05 ...View More

Palumbo, M. C., Dominguez, S., & Dong, H. (2020). Sex differences in hypothalamic-pituitary-adrenal axis regulation after chronic unpredictable stress. Brain and Behavior, 10(4), e01586.

Mouse Anti-FKBP5 Recombinant Antibody (CBXF-1973) (V2LY-0925-LY2089) in WB

Western blot analysis of GST-AIP and GST-FKBP51 pull-down of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105, Bcl-G and their truncated versions lacking five utmost C-terminal residues. ...View More

Bernadotte, A., Kumar, R., Winblad, B., & Pavlov, P. F. (2018). In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network. FEBS Open Bio, 8(11), 1830-1843.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-1973

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 358-457 of human FKBP51.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

FK506 Binding Protein 5

Entrez Gene ID

Human	2289
Mouse	14229
Rat	361810

UniProt ID

Human	Q13451
Mouse	Q64378
Rat	Q5U2T9

Function

Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175).

Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866).

Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).

Biological Process

Chaperone-mediated protein folding Source: UniProtKB
Protein folding Source: ProtInc
Protein peptidyl-prolyl isomerization Source: GO_Central
Response to bacterium Source: Ensembl

Cellular Location

Cytoplasm; Nucleus

PTM

Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1 (PubMed:28147277). Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation (PubMed:28147277).

Kageyama, K., Iwasaki, Y., Watanuki, Y., Niioka, K., & Daimon, M. (2021). Differential effects of Fkbp4 and Fkbp5 on regulation of the proopiomelanocortin gene in murine AtT-20 corticotroph cells. International Journal of Molecular Sciences, 22(11), 5724.

Bierer, L. M., Bader, H. N., Daskalakis, N. P., Lehrner, A., Provençal, N., Wiechmann, T., ... & Yehuda, R. (2020). Intergenerational effects of maternal holocaust exposure on FKBP5 methylation. American Journal of Psychiatry, 177(8), 744-753.

Zannas, A. S., Jia, M., Hafner, K., Baumert, J., Wiechmann, T., Pape, J. C., ... & Binder, E. B. (2019). Epigenetic upregulation of FKBP5 by aging and stress contributes to NF-κB–driven inflammation and cardiovascular risk. Proceedings of the National Academy of Sciences, 116(23), 11370-11379.

Klinger-König, J., Hertel, J., Van der Auwera, S., Frenzel, S., Pfeiffer, L., Waldenberger, M., ... & Grabe, H. J. (2019). Methylation of the FKBP5 gene in association with FKBP5 genotypes, childhood maltreatment and depression. Neuropsychopharmacology, 44(5), 930-938.

Hawn, S. E., Sheerin, C. M., Lind, M. J., Hicks, T. A., Marraccini, M. E., Bountress, K., ... & Amstadter, A. B. (2019). GxE effects of FKBP5 and traumatic life events on PTSD: A meta-analysis. Journal of Affective Disorders, 243, 455-462.

Roberts, S., Keers, R., Breen, G., Coleman, J. R., Jöhren, P., Kepa, A., ... & Wong, C. C. (2019). DNA methylation of FKBP5 and response to exposure‐based psychological therapy. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, 180(2), 150-158.

Ising, M., Maccarrone, G., Brückl, T., Scheuer, S., Hennings, J., Holsboer, F., ... & Lucae, S. (2019). FKBP5 gene expression predicts antidepressant treatment outcome in depression. International journal of molecular sciences, 20(3), 485.

Wang, Q., Shelton, R. C., & Dwivedi, Y. (2018). Interaction between early-life stress and FKBP5 gene variants in major depressive disorder and post-traumatic stress disorder: A systematic review and meta-analysis. Journal of affective disorders, 225, 422-428.

Matosin, N., Halldorsdottir, T., & Binder, E. B. (2018). Understanding the molecular mechanisms underpinning gene by environment interactions in psychiatric disorders: the FKBP5 model. Biological psychiatry, 83(10), 821-830.

Tozzi, L., Farrell, C., Booij, L., Doolin, K., Nemoda, Z., Szyf, M., ... & Frodl, T. (2018). Epigenetic changes of FKBP5 as a link connecting genetic and environmental risk factors with structural and functional brain changes in major depression. Neuropsychopharmacology, 43(5), 1138-1145.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FKBP5 Matched Antibody Pair (447) (CAT#: APMAB-447LY)

FKBP5 Matched Antibody Pair (448) (CAT#: APMAB-448LY)

FKBP5 Matched Antibody Pair (449) (CAT#: APMAB-449LY)

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FKBP5 Matched Antibody Pair (447) (CAT#: APMAB-447LY)

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Isotype control

For research use only. Not intended for any clinical use.

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