Mouse Anti-FKBP8 Recombinant Antibody (CBXF-0718) (V2LY-0925-LY2112)

Name: Mouse Anti-FKBP8 Recombinant Antibody (CBXF-0718)
SKU: V2LY-0925-LY2112
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FKBP8 Recombinant Antibody (CBXF-0718) (V2LY-0925-LY2112) in WB

Western blot shows stable expression of α-SYN and Pin1, CYPA, FKBP12, FKBP38, or FKBP65 after transduction with respective LV. In each lane cell extract containing 10 μg of total protein was loaded. Upper panel, Pin1, CYPA, FKBP12, FKBP38, or FKBP65 expression was visualized in SHSY5Y cells overexpressing α-SYN only (NC) (lanes 1, 3, 5, 7, and 9) or in combination with Pin1 (lane 2), CYPA (lane 4), FKBP38 (lane 6), FKBP65 (lane 8), or FKBP12 (lane 10). Middle panel, stable α-SYN overexpression is shown. Lower panel, α-tubulin is shown as the loading control. Semiquantitative estimates of CYPA, Pin1, FKBP12, FKBP38, and FKBP65 overexpression levels. ...View More

Deleersnijder, A., Van Rompuy, A. S., Desender, L., Pottel, H., Buée, L., Debyser, Z., ... & Gerard, M. (2011). Comparative analysis of different peptidyl-prolyl isomerases reveals FK506-binding protein 12 as the most potent enhancer of α-synuclein aggregation. Journal of Biological Chemistry, 286(30), 26687-26701.

Mouse Anti-FKBP8 Recombinant Antibody (CBXF-0718) (V2LY-0925-LY2112) in IF

Subcellular localization of α-SYN and CYPA, Pin1, FKBP12, FKBP38, or FKBP65. Confocal microscopy of subcellular localization of α-SYN and CYPA (A), Pin1 (B), FKBP12 (C), FKBP38 (D), or FKBP65 (E) in cell lines that stably overexpress the respective PPIases is shown. Co-localization of FKBP38 with Mitotracker Deep Red 633 in FKBP38 overexpression cell line is shown in F. Co-localization of FKBP65 with the ER marker calnexin in FKBP65 overexpression cell line is shown in G. DAPI staining (1) is shown in blue. Immunocytochemical staining for CYPA, Pin1, FKBP12, FKBP38, or FKBP65 is shown in red (2). Immunocytochemical staining for α-SYN and calnexin or Mitotracker Deep Red 633 staining is shown in green (3). Overlap image (4). The bars represent 10 μm. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0718

Application

WB, IP, ELISA, IF

Immunogen

Amino acids 136-355 mapping at the C-terminus of human FKBP8.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

FK506 Binding Protein 8

Entrez Gene ID

23770

UniProt ID

Q14318

Function

Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.

Biological Process

Apoptotic process Source: UniProtKB-KW
Camera-type eye development Source: Ensembl
Cell fate specification Source: Ensembl
Dorsal/ventral neural tube patterning Source: Ensembl
Intracellular signal transduction Source: ProtInc
Multicellular organism growth Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of protein phosphorylation Source: CAFA
Positive regulation of BMP signaling pathway Source: Ensembl
Protein folding Source: MGI
Regulation of gene expression Source: Ensembl
Smoothened signaling pathway Source: Ensembl

Cellular Location

Mitochondrion; Mitochondrion membrane
Isoform 1: Mitochondrion membrane
Isoform 3: Mitochondrion membrane

Topology

Helical: 390-410

PTM

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Zuo, L., Kuo, W. T., Cao, F., Chanez-Paredes, S. D., Zeve, D., Mannam, P., ... & Turner, J. R. (2022). Tacrolimus-binding protein FKBP8 directs myosin light chain kinase-dependent barrier regulation and is a potential therapeutic target in Crohn’s disease. Gut.

Aguilera, M. O., Robledo, E., Melani, M., Wappner, P., & Colombo, M. I. (2022). FKBP8 is a novel molecule that participates in the regulation of the autophagic pathway. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1869(5), 119212.

Lv, S., Zhao, X., Zhang, E., Yan, Y., Ma, X., Li, N., ... & Song, T. (2022). Lysine demethylase KDM1A promotes cell growth via FKBP8–BCL2 axis in hepatocellular carcinoma. Journal of Biological Chemistry, 298(9).

Zhang, J., Yin, Y., Wang, J., Zhang, J., Liu, H., Feng, W., ... & Xu, Y. (2021). Prohibitin regulates mTOR pathway via interaction with FKBP8. Frontiers of Medicine, 15, 448-459.

Yoo, S. M., Yamashita, S. I., Kim, H., Na, D., Lee, H., Kim, S. J., ... & Jung, Y. K. (2020). FKBP8 LIRL‐dependent mitochondrial fragmentation facilitates mitophagy under stress conditions. The FASEB Journal, 34(2), 2944-2957.

Tian, T., Cao, X., Kim, S. E., Lin, Y. L., Steele, J. W., Cabrera, R. M., ... & Lei, Y. (2020). FKBP8 variants are risk factors for spina bifida. Human molecular genetics, 29(18), 3132-3144.

Hsu, F. F., Chou, Y. T., Chiang, M. T., Li, F. A., Yeh, C. T., Lee, W. H., & Chau, L. Y. (2019). Signal peptide peptidase promotes tumor progression via facilitating FKBP8 degradation. Oncogene, 38(10), 1688-1701.

Xu, S. S., Xu, L. G., Yuan, C., Li, S. N., Chen, T., Wang, W., ... & Rao, H. (2019). FKBP8 inhibits virus‐induced RLR‐VISA signaling. Journal of Medical Virology, 91(3), 482-492.

Peng, Y. J., Lee, Y. C., Fu, S. J., Chien, Y. C., Liao, Y. F., Chen, T. Y., ... & Tang, C. Y. (2018). FKBP8 enhances protein stability of the CLC-1 chloride channel at the plasma membrane. International journal of molecular sciences, 19(12), 3783.

Misaka, T., Murakawa, T., Nishida, K., Omori, Y., Taneike, M., Omiya, S., ... & Otsu, K. (2018). FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice. Journal of Molecular and Cellular Cardiology, 114, 93-104.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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