Rabbit Anti-FNTB Recombinant Antibody (
5H4) (V2LY-0725-LY1143)

Name: Rabbit Anti-FNTB Recombinant Antibody (5H4)
SKU: V2LY-0725-LY1143
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-FNTB Recombinant Antibody (5H4) (V2LY-0725-LY1143) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue
All lanes: FNTB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 44 kDa
Observed band size: 49 kDa

Rabbit Anti-FNTB Recombinant Antibody (5H4) (V2LY-0725-LY1143) in IHC

IHC image of V2LY-0725-LY1143 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-FNTB Recombinant Antibody (5H4) (V2LY-0725-LY1143) in IP

Immunoprecipitating FNTB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1143 in K562 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY1143(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

5H4

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human FNTB.

Host Species

Rabbit

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

farnesyltransferase, CAAX box, beta

Entrez Gene ID

2342

UniProt ID

P49356

Function

Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.

Biological Process

Protein farnesylation Source: UniProtKB
Protein prenylation Source: GO_Central

Cellular Location

Microtubule associated complex; Cytosol; Protein farnesyltransferase complex

Bachmann, H. S., Jung, D., Link, T., Arnold, A., Kantelhardt, E., Thomssen, C., ... & Kuhlmann, J. D. (2022). FNTB promoter polymorphisms are independent predictors of survival in patients with triple negative breast cancer. Cancers, 14(3), 468.

Ding, J., Chen, Y. X., Chen, Y., Mou, Y., Sun, X. T., Dai, D. P., ... & Guo, X. (2020). Overexpression of FNTB and the activation of Ras induce hypertrophy and promote apoptosis and autophagic cell death in cardiomyocytes. Journal of Cellular and Molecular Medicine, 24(16), 8998-9011.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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