Mouse Anti-FNTB Recombinant Antibody (CBXF-0803) (CBMAB-F3193-CQ)

Name: Mouse Anti-FNTB Recombinant Antibody (CBXF-0803)
SKU: CBMAB-F3193-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FNTB Recombinant Antibody (CBXF-0803) (CBMAB-F3193-CQ) in Immunoblot

Immunoblots of co-expression and GST pull-downs. A and B (co-expression and IMAC), P (pellet), CE (crude extract), FT (flowthrough), wash 1 and 3 (W1, W3) and the 1st three elution fraction (E1, E2 and E3) are shown. The membranes were incubated with anti-His antibody (red) and anti-FNTA-antibody (green); C and D GST-pulldown of truncated human and rat FTα-GST with FTβ, fractions: beads (B), supernatant (SN), wash of the beads (WB), interaction (I), wash after interaction (WI) and elution 1 and 2 (E1, E2) The membranes were incubated with anti-His antibody (red) and anti-GST antibody (green). All samples were run on 12% SDS-PAGE and blotted by semi-dry blot ...View More

Hagemann, A., Tasillo, S., Aydin, A., Kehrenberg, M. C. A., & Bachmann, H. S. (2022). Impact of a conserved N-terminal proline-rich region of the α-subunit of CAAX-prenyltransferases on their enzyme properties. Cell Communication and Signaling, 20(1), 118.

Mouse Anti-FNTB Recombinant Antibody (CBXF-0803) (CBMAB-F3193-CQ) in WB

Western blot analysis on pancreas lysates from Ela-KrasG12D/+ mice treated (n = 3) or not (n = 3) with cerulein for 1 week. ...View More

Assi, M., Pirlot, B., Stroobant, V., Thissen, J. P., & Jacquemin, P. (2020). A Novel KRAS antibody highlights a regulation mechanism of post-translational modifications of KRAS during Tumorigenesis. International journal of molecular sciences, 21(17), 6361.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0803

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 138-437 of human FTβ.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

farnesyltransferase, CAAX box, beta

Entrez Gene ID

Human	2342
Rat	64511

UniProt ID

Human	P49356
Rat	Q02293

Alternative Names

Farnesyltransferase, CAAX Box, Beta; Ras Proteins Prenyltransferase Subunit Beta; CAAX Farnesyltransferase Subunit Beta; EC 2.5.1.58; FTase-Beta; Protein Farnesyltransferase Subunit Beta; FPTB;

Function

Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.

Biological Process

Protein farnesylation Source: UniProtKB
Protein prenylation Source: GO_Central

Cellular Location

Microtubule associated complex; Cytosol; Protein farnesyltransferase complex

Bachmann, H. S., Jung, D., Link, T., Arnold, A., Kantelhardt, E., Thomssen, C., ... & Kuhlmann, J. D. (2022). FNTB promoter polymorphisms are independent predictors of survival in patients with triple negative breast cancer. Cancers, 14(3), 468.

Ding, J., Chen, Y. X., Chen, Y., Mou, Y., Sun, X. T., Dai, D. P., ... & Guo, X. (2020). Overexpression of FNTB and the activation of Ras induce hypertrophy and promote apoptosis and autophagic cell death in cardiomyocytes. Journal of Cellular and Molecular Medicine, 24(16), 8998-9011.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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