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Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561)

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Published Data
V2LY-0925-LY2561-1
Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561) in WB
PSMA gene & protein expression among canine uroepithelial carcinoma cell lines. (A) PSMA gene expression patterns among canine uroepithelial carcinoma cell lines. Error bars represent RQ min and max values from a representative biologic replicate. (B) PSMA western blot image with known human control cell lines (LNCaP +, PC3-), and (C) PSMA protein expression normalized to β-actin loading control. Within bar graphs, grey represents canine PCA cell lines and red represents canine iUC cell lines. ...View More
Berry, M. R., Fadl-Alla, B. A., Samuelson, J., Rosol, T. J., & Fan, T. M. (2022). Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection. BMC Veterinary Research, 18(1), 441.
V2LY-0925-LY2561-2
Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561) in IHC
Immunohistochemical detection of PSMA among canine uroepithelial carcinoma cell lines and representative patient samples. (A) PSMA IHC of known human reference control cell lines (LNCaP+, PC3-). (B) PSMA IHC among canine uroepithelial cell lines with grey labeled (top row) representing PCA cell lines and red labelled (bottom row) representing iUC cell lines. (C) PSMA IHC of archived iUC patient sample (red labelled, left) and carcinomas of the prostate (grey labelled, right). ...View More
Berry, M. R., Fadl-Alla, B. A., Samuelson, J., Rosol, T. J., & Fan, T. M. (2022). Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection. BMC Veterinary Research, 18(1), 441.
V2LY-0925-LY2561-3
Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561) in IHC
Characterization of PSMA in cHSA tumor samples.
Spontaneously-arising cHSA tumor samples involving the spleen, liver, and lung tissues, with representative (A) histology by H&E and PSMA immunohistochemistry (Score 3; magnification 400x), and comparison (B) of staining properties for cHSA by CD31 and PSMA (magnification 100x). ...View More
Dowling, M., Samuelson, J., Fadl-Alla, B., Pondenis, H. C., Byrum, M., Barger, A. M., & Fan, T. M. (2019). Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. Plos one, 14(1), e0210297.
V2LY-0925-LY2561-4
Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561) in IF and WB
Antibody validation for canine PSMA protein expression.
Validation of antibodies for detecting PSMA in human and canine cell lines by (A) confocal fluorescent microscopy, and (B) western blot analysis. Positive and negative controls for canine and human include (CPA and ACE-1) and (LNCaP and PC3), respectively. ...View More
Dowling, M., Samuelson, J., Fadl-Alla, B., Pondenis, H. C., Byrum, M., Barger, A. M., & Fan, T. M. (2019). Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. Plos one, 14(1), e0210297.
V2LY-0925-LY2561-5
Mouse Anti-FOLH1 Recombinant Antibody (CBXF-2346) (V2LY-0925-LY2561) in IF
Immunofluorescence staining of U87 tumors. Nuclear staining (DAPI) is shown in blue, antibody staining in green, and tumor borders are outlined in white. Strong reactive astrocytosis (GFAP; a) is visible in the peritumoral region with few astrocytes at the tumor border. Specific staining of blood vessels (vWF; b) is detectable, which is similar within tumor tissue and in the peritumoral region. Few activated microglia (CD11b; c) can be observed in the peritumoral region and inside the tumor. PSMA staining using the antibody NBP1-45057 is positive in vessel-like structures in the tumor center (d) but not outside the tumor. ...View More
Oliveira, D., Stegmayr, C., Heinzel, A., Ermert, J., Neumaier, B., Shah, N. J., ... & Willuweit, A. (2020). High uptake of 68Ga-PSMA and 18F-DCFPyL in the peritumoral area of rat gliomas due to activated astrocytes. EJNMMI research, 10(1), 55.
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBXF-2346
Application
IF, WB, IHC
Immunogen
Recombinant fragment of human GCPII (aa 44-750).
Host Species
Mouse
Specificity
Human, Mouse, Rat, Pig, Dog
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1 µg/ml
IHC1:200
IF(ICC)1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Glutamate carboxypeptidase 2
Entrez Gene ID
Human2346
Mouse53320
Rat85309
Dog476775
UniProt ID
HumanQ04609
MouseO35409
RatP70627
DogA0A5F4CAY7
Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.

Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Biological Process
C-terminal protein deglutamylation Source: BHF-UCL
Proteolysis Source: BHF-UCL
Cellular Location
Cell membrane
Isoform PSMA': Cytoplasm
Topology
Cytoplasmic: 1-19
Helical: 20-43
Extracellular: 44-750
PTM
The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.

Sun, D., Zhang, A., Gao, B., Zou, L., Huang, H., Zhao, X., & Xu, D. (2022). Identification of Alternative Splicing-Related Genes CYB561 and FOLH1 in the Tumor-Immune Microenvironment for Endometrial Cancer Based on TCGA Data Analysis. Frontiers in Genetics, 13.

Ramirez‐Fort, M. K., Meier‐Schiesser, B., Lachance, K., Mahase, S. S., Church, C. D., Niaz, M. J., ... & Bander, N. H. (2021). Folate hydrolase‐1 (FOLH1) is a novel target for antibody‐based brachytherapy in Merkel cell carcinoma. Skin health and disease, 1(1), e9.

Zink, C. F., Barker, P. B., Sawa, A., Weinberger, D. R., Wang, M., Quillian, H., ... & Bigos, K. L. (2020). Association of missense mutation in FOLH1 with decreased NAAG levels and impaired working memory circuitry and cognition. American Journal of Psychiatry, 177(12), 1129-1139.

Bahler, C. D., Johnson, M. M., Davicioni, E., Zhang, J., Cheng, L., Green, M. A., & Koch, M. O. (2020). Predictors of Prostate-specific Membrane Antigen (PSMA/FOLH1) Expression in a Genomic Database. Urology, 144, 117-122.

Kwan, E. M., To, S. Q., Fettke, H. C., Docanto, M. M., Bukczynska, P., Mant, A. M., ... & Azad, A. (2019). Whole blood FOLH1 mRNA expression and treatment response in metastatic castration-resistant prostate cancer (mCRPC).

Šilhavý, J., Marková, I., Hüttl, M., Malínská, H., Kazdová, L., Liška, F., ... & Oliyarnyk, O. (2018). Dissecting the role of Folr1 and Folh1 genes in the pathogenesis of metabolic syndrome in spontaneously hypertensive rats. Physiological research, 67(4), 657-662.

Paul, S., Sadhukhan, S., Munian, D., Bankura, B., & Das, M. (2018). Association of FOLH1, DHFR, and MTHFR gene polymorphisms with susceptibility of Neural Tube Defects: A case control study from Eastern India. Birth Defects Research, 110(14), 1129-1138.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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