Mouse Anti-FPR1 Recombinant Antibody (CBXF-2667) (V2LY-1225-LY71)

Name: Mouse Anti-FPR1 Recombinant Antibody (CBXF-2667)
SKU: V2LY-1225-LY71
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FPR1 Recombinant Antibody (CBXF-2667) (V2LY-1225-LY71) in ICC

left: representative immunocytochemistry staining of U87 cells and transfected HEK293T cells with FPR subtype-specific antibodies (red) and nuclei staining (blue). For visibility, brightness and contrast were adjusted for U87 cells and HEK293T cells differently; for absolute intensity comparison pictures with equal settings for exposure time, brightness and contrast pictures are shown in Figure S5. Scale bars indicate 100 μm. Right: Quantification of the mean FPR staining in U87 cells (green) in comparison to FPR-transfected HEK293T cells (red). Analysis was performed on images acquired with the same settings. n = 2, N = 5 for U87 cells and n = 2, N = 2 for HEK293T cells. ...View More

Busch, L., Al Taleb, Z., Tsai, Y. L., Nguyen, V. T. T., Lu, Q., Synatschke, C. V., ... & Bufe, B. (2022). Amyloid beta and its naturally occurring N-terminal variants are potent activators of human and mouse formyl peptide receptor 1. Journal of Biological Chemistry, 298(12), 102642.

Mouse Anti-FPR1 Recombinant Antibody (CBXF-2667) (V2LY-1225-LY71) in ICC

Left, representative images showing immunostainings of hFPR1 (green) and the corresponding mock staining using an isotype KLH antibody as negative control. Similar results were obtained in three independent experiments. Red, cell nuclei; scale bar, 50 μm. Right, percentage of cells expressing hFPR1 or the monocyte marker hCD14 (n = 3). As isotype controls, we used a KLH antibody for hFPR1 and a MOPC-21 antibody for hCD14. ...View More

Bufe, B., Schumann, T., Kappl, R., Bogeski, I., Kummerow, C., Podgorska, M., ... & Zufall, F. (2015). Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens. Journal of Biological Chemistry, 290(12), 7369-7387.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2667

Application

Immunogen

Human FPR1 (Met1-Lys350).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

Application	Note
FC	2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Formyl Peptide Receptor 1

Entrez Gene ID

2357

UniProt ID

P21462

Function

High affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors (PubMed:2161213, PubMed:2176894, PubMed:10514456, PubMed:15153520).

Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release (PubMed:2161213, PubMed:1712023, PubMed:15153520).

This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:1712023, PubMed:10514456).

Receptor for TAFA4, mediates its effects on chemoattracting macrophages, promoting phagocytosis and increasing ROS release (PubMed:25109685).

Biological Process

Activation of MAPK activity Source: ProtInc
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Chemotaxis Source: ProtInc
Complement receptor mediated signaling pathway Source: GO_Central
G protein-coupled receptor signaling pathway Source: ProtInc
Inflammatory response Source: GO_Central
Nitric oxide mediated signal transduction Source: ProtInc
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: MGI
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Cell membrane. Internalizes in presence of its ligand, TAFA4.

Topology

Extracellular: 1-27
Helical: 28-50
Cytoplasmic: 51-61
Helical: 62-83
Extracellular: 84-100
Helical: 101-121
Cytoplasmic: 122-140
Helical: 141-162
Extracellular: 163-205
Helical: 206-226
Cytoplasmic: 227-242
Helical: 243-266
Extracellular: 267-285
Helical: 286-305
Cytoplasmic: 306-350

PTM

Phosphorylated; which is necessary for desensitization.

Zhuang, Y., Wang, L., Guo, J., Sun, D., Wang, Y., Liu, W., ... & Zhang, C. (2022). Molecular recognition of formylpeptides and diverse agonists by the formylpeptide receptors FPR1 and FPR2. Nature communications, 13(1), 1054.

Vecchi, L., Mota, S. T. S., Zóia, M. A. P., Martins, I. C., de Souza, J. B., Santos, T. G., ... & Araújo, T. G. (2022). Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment. Cells, 11(10), 1705.

Liotti, F., Marotta, M., Sorriento, D., Pagliuca, C., Caturano, V., Mantova, G., ... & Prevete, N. (2022). Probiotic Lactobacillus rhamnosus GG (LGG) restrains the angiogenic potential of colorectal carcinoma cells by activating a proresolving program via formyl peptide receptor 1. Molecular Oncology, 16(16), 2959-2980.

Korinek, M., Hsieh, P. S., Chen, Y. L., Hsieh, P. W., Chang, S. H., Wu, Y. H., & Hwang, T. L. (2021). Randialic acid B and tomentosolic acid block formyl peptide receptor 1 in human neutrophils and attenuate psoriasis-like inflammation in vivo. Biochemical Pharmacology, 190, 114596.

Chiang, S. F., Huang, K. C. Y., Chen, W. T. L., Chen, T. W., Ke, T. W., & Chao, K. C. (2021). Polymorphism of formyl peptide receptor 1 (FPR1) reduces the therapeutic efficiency and antitumor immunity after neoadjuvant chemoradiotherapy (CCRT) treatment in locally advanced rectal cancer. Cancer Immunology, Immunotherapy, 70(10), 2937-2950.

Li, Z., Li, Y., Han, J., Zhu, Z., Li, M., Liu, Q., ... & Shi, F. D. (2021). Formyl peptide receptor 1 signaling potentiates inflammatory brain injury. Science translational medicine, 13(605), eabe9890.

Fusco, R., Gugliandolo, E., Siracusa, R., Scuto, M., Cordaro, M., D’Amico, R., ... & Di Paola, R. (2020). Formyl peptide receptor 1 signaling in acute inflammation and neural differentiation induced by traumatic brain injury. Biology, 9(9), 238.

Nishiguchi, T., Yoshimura, H., Kasai, R. S., Fujiwara, T. K., & Ozawa, T. (2020). Synergetic roles of formyl peptide receptor 1 oligomerization in ligand-induced signal transduction. ACS Chemical Biology, 15(9), 2577-2587.

Li, S. Q., Yu, Y., Zhang, Y., Sun, Y. P., Li, X. X., & Su, N. (2020). The role of formyl peptide receptor 1 gene polymorphisms in human colorectal cancer. Journal of Cancer, 11(12), 3580.

Fusco, R., D’amico, R., Cordaro, M., Gugliandolo, E., Siracusa, R., Peritore, A. F., ... & Di Paola, R. (2018). Absence of formyl peptide receptor 1 causes endometriotic lesion regression in a mouse model of surgically-induced endometriosis. Oncotarget, 9(59), 31355.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-FPR1 Recombinant Antibody (CBXF-2667) (CAT#: CBMAB-F0149-CQ)

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Isotype control

For research use only. Not intended for any clinical use.

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