Mouse Anti-FUT1 Recombinant Antibody (CBXF-2523) (V2LY-1225-LY276)

Name: Mouse Anti-FUT1 Recombinant Antibody (CBXF-2523)
SKU: V2LY-1225-LY276
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2523

Application

WB, IP, IF, FC

Immunogen

H antigen on human red blood cells.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgM

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fucosyltransferase 1 (H Blood Group)

Entrez Gene ID

2523

UniProt ID

P19526

Function

Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the terminal galactose residue of glycoconjugates through an alpha(1,2) linkage leading to H antigen synthesis that is an intermediate substrate in the synthesis of ABO blood group antigens (PubMed:2118655).

H antigen is essential for maturation of the glomerular layer of the main olfactory bulb, in cell migration and early cell-cell contacts during tumor associated angiogenesis (PubMed:18205178).

Preferentially fucosylates soluble lactose and to a lesser extent fucosylates glycolipids gangliosides GA1 and GM1a (By similarity).

Biological Process

Carbohydrate metabolic process Source: ProtInc
Fucosylation Source: UniProtKB
L-fucose catabolic process Source: UniProtKB
Olfactory bulb development Source: UniProtKB
Oligosaccharide biosynthetic process Source: Reactome
Positive regulation of cell-matrix adhesion Source: UniProtKB
Positive regulation of endothelial cell-matrix adhesion via fibronectin Source: UniProtKB
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of sprouting angiogenesis Source: UniProtKB
Protein glycosylation Source: UniProtKB
Regulation of cell adhesion Source: UniProtKB
Regulation of endothelial cell proliferation Source: UniProtKB

Cellular Location

Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.

Topology

Cytoplasmic: 1-8
Helical: 9-25
Lumenal: 26-365

Rattanapan, Y., Charong, N., Narkpetch, S., & Chareonsirisuthigul, T. (2022). Genotyping of the rare Para-Bombay blood group in southern Thailand. Hematology, Transfusion and Cell Therapy.

Lei, H., Shen, Y., Wang, Y., Su, N., Wang, X., & Cai, X. (2021). A Para-Bombay Blood Group Case Associated with a Novel FUT1 Mutation c. 361G> A. Transfusion Medicine and Hemotherapy, 48(4), 254-258.

Fan, R., Han, X., Gong, Y., He, L., Xue, Z., Yang, Y., ... & Zhang, J. (2021). Alterations of fucosyltransferase genes and fucosylated glycans in gastric epithelial cells infected with Helicobacter pylori. Pathogens, 10(2), 168.

Matzhold, E. M., Wagner, T., Drexler, C., Schönbacher, M., & Körmöczi, G. F. (2020). Aberrant ABO B phenotype with irregular anti-B caused by a para-Bombay FUT1 mutation. Transfusion Medicine and Hemotherapy, 47(1), 94-97.

Lai, T. Y., Chen, I. J., Lin, R. J., Liao, G. S., Yeo, H. L., Ho, C. L., ... & Yu, A. L. (2019). Fucosyltransferase 1 and 2 play pivotal roles in breast cancer cells. Cell death discovery, 5(1), 74.

Scharberg, E. A., Olsen, C., & Bugert, P. (2019). An update on the H blood group system. Immunohematology, 35(2), 67-68.

Colston, J. M., Francois, R., Pisanic, N., Peñataro Yori, P., McCormick, B. J., Olortegui, M. P., ... & Kosek, M. N. (2019). Effects of child and maternal histo-blood group antigen status on symptomatic and asymptomatic enteric infections in early childhood. The Journal of infectious diseases, 220(1), 151-162.

Lin, J., Chen, R., Zhu, S., Qiu, X., Huang, Y., & Wang, X. (2019). Sequence analysis of α-(1, 2)-fucosyltransferase gene in nine Chinese individuals with Para-Bombay phenotype. Gene, 685, 21-23.

Chen, J., Su, Z., Zhang, C., Li, F., Hwu, P., Wang, Z., ... & Zhou, D. (2019). Normal development and fertility of Fut1, Fut2, and Sec1 triple knockout mice. bioRxiv, 615070.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-FUT1 Recombinant Antibody (CBXF-2523) (CAT#: CBMAB-F1905-CQ)

Related Products

Mouse Anti-FUT1 Recombinant Antibody (CBXF-2523) (CAT#: CBMAB-F1905-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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