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Mouse Anti-FXYD2 Recombinant Antibody (1C3-B3) (CBMAB-A3246-LY)

The product is antibody recognizes FXYD2. The antibody 1C3-B3 immunoassay techniques such as: WB, ELISA.
See all FXYD2 antibodies
Published Data
Fig.1 FXYD2 is detected in subpopulations of sensory neurons of small- (black arrows) and medium-diameters (black arrowheads).
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Fig.2 Representative images of immunofluorescent staining using an anti-Fxyd2 antibody.
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Fig.3 Normal human pancreas sections were analysed by immunohistochemistry with a monoclonal antibody directed against FXYD2.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1C3-B3
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
FXYD2 (AAH05302.1, 1 a.a. ~ 64 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
FXYD domain containing ion transport regulator 2
Introduction
This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The Type III integral membrane protein encoded by this gene is the gamma subunit of the Na,K-ATPase present on the plasma membrane. Although the Na,K-ATPase does not depend on the gamma subunit to be functional, it is thought that the gamma subunit modulates the enzyme's activity by inducing ion channel activity. Mutations in this gene have been associated with renal hypomagnesaemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
486
UniProt ID
P54710
Alternative Names
ATP1G1; HOMG2; MGC12372
Function
May be involved in forming the receptor site for cardiac glycoside binding or may modulate the transport function of the sodium ATPase.
Biological Process
Positive regulation of P-type sodium:potassium-exchanging transporter activity Source: ARUK-UCL
Potassium ion import across plasma membrane Source: BHF-UCL
Regulation of sodium ion transmembrane transporter activity Source: GO_Central
Sodium ion export across plasma membrane Source: BHF-UCL
Transmembrane transport Source: BHF-UCL
Cellular Location
Membrane
Involvement in disease
Hypomagnesemia 2 (HOMG2):
A disorder due to primary renal wasting of magnesium. Plasma levels of other electrolytes are normal. The only abnormality found, in addition to low magnesium levels, is lowered renal excretion of calcium resulting in hypocalciuria.
Topology
Helical: 29-46

Zhang, Z., Tang, Y., Li, L., Yang, W., Xu, Y., Zhou, J., ... & Gong, K. (2022). Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma. Journal of Immunology Research, 2022.

Fabri, L. M., Moraes, C. M., Costa, M. I., Garçon, D. P., Fontes, C. F., Pinto, M. R., ... & Leone, F. A. (2022). Salinity-dependent modulation by protein kinases and the FXYD2 peptide of gill (Na+, K+)-ATPase activity in the freshwater shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). Biochimica et Biophysica Acta (BBA)-Biomembranes, 1864(10), 183982.

Kaijia, Z. (2022). FXYD2 mRNA expression represents a new independent factor that affects survival of glioma patients and predicts chemosensitivity of patients to temozolomide. Brain Tumor Research and Treatment, 10(Suppl).

Chung, Y. D., Liu, T. H., Liang, Y. L., Lin, C. N., Hsu, K. F., & Lee, G. B. (2021). An integrated microfluidic platform for detection of ovarian clear cell carcinoma mRNA biomarker FXYD2. Lab on a Chip, 21(13), 2625-2632.

Zhou, K., Jiang, T., Liu, Y., Zhao, Z., Huang, L., & Li, G. (2021). FXYD2 mRNA expression represents a new independent factor that affects survival of glioma patients and predicts chemosensitivity of patients to temozolomide. BMC neurology, 21(1), 1-13.

Chung, Y. D., Chuang, Y. J., Lin, C. N., Hsu, K. F., & Lee, G. B. (2021, January). An Integrated Microfluidic Platform to Detect FXYD2 RNA Expression in Ascites for Diagnosis of Ovarian Clear Cell

Leone, F. A., Lucena, M. N., Fabri, L. M., Garçon, D. P., Fontes, C. F., Faleiros, R. O., ... & McNamara, J. C. (2020). Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na+, K+)-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 250, 110507.

Leone, F. A., Lucena, M. N., Fabri, L. M., Garçon, D. P., Fontes, C. F., Faleiros, R. O., ... & McNamara, J. C. (2020). Effect of salinity on modulation by ATP, protein kinases and FXYD2 peptide of gill (Na+, K+)-ATPase activity in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae). bioRxiv, 2020-04.

Liu, T. H., Lin, C. N., Hsu, K. F., & Lee, G. B. (2019). A microfluidic platform for diagnosis of ovarian clear cell carcinoma via quantification of fxyd2 gene. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 622-623). Chemical and Biological Microsystems Society.

Mayan, H., Farfel, Z., & Karlish, S. J. (2018). Renal Mg handling, FXYD 2 and the central role of the Na, K‐ATP ase. Physiological reports, 6(17), e13843.

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For research use only. Not intended for any clinical use.

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