Mouse Anti-FXYD2 Recombinant Antibody (1C3-B3) (CBMAB-A3246-LY)

Name: Mouse Anti-FXYD2 Recombinant Antibody (1C3-B3)
SKU: CBMAB-A3246-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1C3-B3

Application

WB, ELISA

Immunogen

FXYD2 (AAH05302.1, 1 a.a. ~ 64 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

FXYD domain containing ion transport regulator 2

Introduction

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The Type III integral membrane protein encoded by this gene is the gamma subunit of the Na,K-ATPase present on the plasma membrane. Although the Na,K-ATPase does not depend on the gamma subunit to be functional, it is thought that the gamma subunit modulates the enzyme's activity by inducing ion channel activity. Mutations in this gene have been associated with renal hypomagnesaemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Entrez Gene ID

486

UniProt ID

P54710

Alternative Names

ATP1G1; HOMG2; MGC12372

Function

May be involved in forming the receptor site for cardiac glycoside binding or may modulate the transport function of the sodium ATPase.

Biological Process

Positive regulation of P-type sodium:potassium-exchanging transporter activity Source: ARUK-UCL
Potassium ion import across plasma membrane Source: BHF-UCL
Regulation of sodium ion transmembrane transporter activity Source: GO_Central
Sodium ion export across plasma membrane Source: BHF-UCL
Transmembrane transport Source: BHF-UCL

Cellular Location

Membrane

Involvement in disease

Hypomagnesemia 2 (HOMG2):
A disorder due to primary renal wasting of magnesium. Plasma levels of other electrolytes are normal. The only abnormality found, in addition to low magnesium levels, is lowered renal excretion of calcium resulting in hypocalciuria.

Topology

Helical: 29-46

Zhang, Z., Tang, Y., Li, L., Yang, W., Xu, Y., Zhou, J., ... & Gong, K. (2022). Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma. Journal of Immunology Research, 2022.

Fabri, L. M., Moraes, C. M., Costa, M. I., Garçon, D. P., Fontes, C. F., Pinto, M. R., ... & Leone, F. A. (2022). Salinity-dependent modulation by protein kinases and the FXYD2 peptide of gill (Na+, K+)-ATPase activity in the freshwater shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). Biochimica et Biophysica Acta (BBA)-Biomembranes, 1864(10), 183982.

Kaijia, Z. (2022). FXYD2 mRNA expression represents a new independent factor that affects survival of glioma patients and predicts chemosensitivity of patients to temozolomide. Brain Tumor Research and Treatment, 10(Suppl).

Chung, Y. D., Liu, T. H., Liang, Y. L., Lin, C. N., Hsu, K. F., & Lee, G. B. (2021). An integrated microfluidic platform for detection of ovarian clear cell carcinoma mRNA biomarker FXYD2. Lab on a Chip, 21(13), 2625-2632.

Zhou, K., Jiang, T., Liu, Y., Zhao, Z., Huang, L., & Li, G. (2021). FXYD2 mRNA expression represents a new independent factor that affects survival of glioma patients and predicts chemosensitivity of patients to temozolomide. BMC neurology, 21(1), 1-13.

Chung, Y. D., Chuang, Y. J., Lin, C. N., Hsu, K. F., & Lee, G. B. (2021, January). An Integrated Microfluidic Platform to Detect FXYD2 RNA Expression in Ascites for Diagnosis of Ovarian Clear Cell

Leone, F. A., Lucena, M. N., Fabri, L. M., Garçon, D. P., Fontes, C. F., Faleiros, R. O., ... & McNamara, J. C. (2020). Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na+, K+)-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 250, 110507.

Leone, F. A., Lucena, M. N., Fabri, L. M., Garçon, D. P., Fontes, C. F., Faleiros, R. O., ... & McNamara, J. C. (2020). Effect of salinity on modulation by ATP, protein kinases and FXYD2 peptide of gill (Na+, K+)-ATPase activity in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae). bioRxiv, 2020-04.

Liu, T. H., Lin, C. N., Hsu, K. F., & Lee, G. B. (2019). A microfluidic platform for diagnosis of ovarian clear cell carcinoma via quantification of fxyd2 gene. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 622-623). Chemical and Biological Microsystems Society.

Mayan, H., Farfel, Z., & Karlish, S. J. (2018). Renal Mg handling, FXYD 2 and the central role of the Na, K‐ATP ase. Physiological reports, 6(17), e13843.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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