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Mouse Anti-FXYD3 Recombinant Antibody (4E3) (CBMAB-F2760-CQ)

This product is a mouse antibody that recognizes FXYD3. The antibody 4E3 can be used for immunoassay techniques such as: WB, IHC, IF, IHC-P.
See all FXYD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4E3
Antibody Isotype
IgG2a
Application
WB, IHC, IF, IHC-P

Basic Information

Immunogen
Full length human recombinant protein of human FXYD3 (NP_005962)
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
FXYD Domain Containing Ion Transport Regulator 3
Introduction
This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Entrez Gene ID
UniProt ID
Alternative Names
FXYD Domain Containing Ion Transport Regulator 3; Sodium/Potassium-Transporting ATPase Subunit FXYD3; FXYD Domain-Containing Ion Transport Regulator 3; Chloride Conductance Inducer Protein Mat-8; Mammary Tumor 8 KDa Protein; PLML; MAT8; Phospholemman-Like Protein; Phospholemman-Like;
Function
Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell (PubMed:17077088).

Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed:21454534).

Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes (PubMed:7836447).

Isoform 1:
Decreases the apparent K+ and Na+ affinity of the sodium/potassium-transporting ATPase over a large range of membrane potentials.

Isoform 2:
Decreases the apparent K+ affinity of the sodium/potassium-transporting ATPase only at slightly negative and positive membrane potentials and increases the apparent Na+ affinity over a large range of membrane potentials.
Biological Process
Chloride transport Source: ProtInc
Potassium ion transport Source: UniProtKB-KW
Regulation of sodium ion transmembrane transporter activity Source: GO_Central
Sodium ion transport Source: UniProtKB-KW
Cellular Location
Cell membrane
Topology
Extracellular: 21-38
Helical: 39-59
Cytoplasmic: 60-87
PTM
Glutathionylated.

Yang, W., He, R., Qu, H., Lian, W., Xue, Y., Wang, T., ... & Wang, Q. (2023). FXYD3 enhances IL-17A signaling to promote psoriasis by competitively binding TRAF3 in keratinocytes. Cellular & Molecular Immunology, 1-13.

Yonekura, S., & Ueda, K. (2022). FXYD3 Expression Predicts Poor Prognosis in Renal Cell Carcinoma with Immunosuppressive Tumor Microenvironment. Cancers, 14(15), 3596.

Cano Portillo, C., Villacreses, R., Thurman, A. L., Pezzulo, A. A., Zabner, J., & Thornell, I. M. (2022). FXYD3 facilitates Na+ and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase. American Journal of Physiology-Cell Physiology, 323(4), C1044-C1051.

Peng, Y., Zeng, X., Lian, M., & Wang, Y. (2022). FXYD3 promotes the proliferation, migration, and invasion of pancreatic cancer cells by regulating the cGMP-PKG signaling pathway. Molecular & Cellular Toxicology, 1-11.

Ma, Y. S., Wu, T. M., Qian, B., Liu, Y. S., Ding, H., Fan, M. M., ... & Fu, D. (2021). KDM5A silencing transcriptionally suppresses the FXYD3‐PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR‐433 up‐regulation. Journal of Cellular and Molecular Medicine, 25(8), 4040-4052.

Liu, J., Feng, Y., Zeng, X., He, M., Gong, Y., & Liu, Y. (2021). Extracellular vesicles‐encapsulated let‐7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. Journal of Cellular and Molecular Medicine, 25(4), 1911-1926.

Hsu, Y. P., Yang, H. W., Li, N. S., Chen, Y. T., Pang, H. H., & Pang, S. T. (2020). Instrument-Free Detection of FXYD3 Using Vial-Based Immunosensor for Earlier and Faster Urothelial Carcinoma Diagnosis. ACS sensors, 5(4), 928-935.

Peng, X., Gao, J., Cai, C., & Zhang, Y. (2020). LncRNA LINC01503 aggravates the progression of cervical cancer through sponging miR-342-3p to mediate FXYD3 expression. Bioscience reports, 40(6).

Xue, Y., Lai, L., Lian, W., Tu, X., Zhou, J., Dong, P., ... & Wang, Q. (2019). SOX9/FXYD3/Src Axis Is Critical for ER+ Breast Cancer Stem Cell FunctionFXYD3 Is Critical for Breast Cancer Stem Cells. Molecular Cancer Research, 17(1), 238-249.

Wang, L. J., Li, Q. J., Le, Y., Ouyang, H. Y., He, M. K., Yu, Z. S., ... & Shi, M. (2018). Prognostic significance of sodium-potassium ATPase regulator, FXYD3, in human hepatocellular carcinoma. Oncology Letters, 15(3), 3024-3030.

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For research use only. Not intended for any clinical use.

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