Mouse Anti-FXYD3 Recombinant Antibody (CBXF-3612) (CBMAB-F2842-CQ)

Name: Mouse Anti-FXYD3 Recombinant Antibody (CBXF-3612)
SKU: CBMAB-F2842-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-3612

Application

WB, IF, IHC

Immunogen

Full length human recombinant protein of FXYD3.

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:100
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA, glycerol

Preservative

Sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

FXYD Domain Containing Ion Transport Regulator 3

Introduction

This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Entrez Gene ID

5349

UniProt ID

Q14802

Alternative Names

FXYD Domain Containing Ion Transport Regulator 3; Sodium/Potassium-Transporting ATPase Subunit FXYD3; FXYD Domain-Containing Ion Transport Regulator 3; Chloride Conductance Inducer Protein Mat-8; Mammary Tumor 8 KDa Protein; PLML; MAT8; Phospholemman-Like Protein; Phospholemman-Like;

Function

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell (PubMed:17077088).

Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed:21454534).

Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes (PubMed:7836447).

Isoform 1:
Decreases the apparent K+ and Na+ affinity of the sodium/potassium-transporting ATPase over a large range of membrane potentials.

Isoform 2:
Decreases the apparent K+ affinity of the sodium/potassium-transporting ATPase only at slightly negative and positive membrane potentials and increases the apparent Na+ affinity over a large range of membrane potentials.

Biological Process

Chloride transport Source: ProtInc
Potassium ion transport Source: UniProtKB-KW
Regulation of sodium ion transmembrane transporter activity Source: GO_Central
Sodium ion transport Source: UniProtKB-KW

Cellular Location

Cell membrane

Topology

Extracellular: 21-38
Helical: 39-59
Cytoplasmic: 60-87

PTM

Glutathionylated.

Yang, W., He, R., Qu, H., Lian, W., Xue, Y., Wang, T., ... & Wang, Q. (2023). FXYD3 enhances IL-17A signaling to promote psoriasis by competitively binding TRAF3 in keratinocytes. Cellular & Molecular Immunology, 1-13.

Yonekura, S., & Ueda, K. (2022). FXYD3 Expression Predicts Poor Prognosis in Renal Cell Carcinoma with Immunosuppressive Tumor Microenvironment. Cancers, 14(15), 3596.

Cano Portillo, C., Villacreses, R., Thurman, A. L., Pezzulo, A. A., Zabner, J., & Thornell, I. M. (2022). FXYD3 facilitates Na+ and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase. American Journal of Physiology-Cell Physiology, 323(4), C1044-C1051.

Peng, Y., Zeng, X., Lian, M., & Wang, Y. (2022). FXYD3 promotes the proliferation, migration, and invasion of pancreatic cancer cells by regulating the cGMP-PKG signaling pathway. Molecular & Cellular Toxicology, 1-11.

Ma, Y. S., Wu, T. M., Qian, B., Liu, Y. S., Ding, H., Fan, M. M., ... & Fu, D. (2021). KDM5A silencing transcriptionally suppresses the FXYD3‐PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR‐433 up‐regulation. Journal of Cellular and Molecular Medicine, 25(8), 4040-4052.

Liu, J., Feng, Y., Zeng, X., He, M., Gong, Y., & Liu, Y. (2021). Extracellular vesicles‐encapsulated let‐7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. Journal of Cellular and Molecular Medicine, 25(4), 1911-1926.

Hsu, Y. P., Yang, H. W., Li, N. S., Chen, Y. T., Pang, H. H., & Pang, S. T. (2020). Instrument-Free Detection of FXYD3 Using Vial-Based Immunosensor for Earlier and Faster Urothelial Carcinoma Diagnosis. ACS sensors, 5(4), 928-935.

Peng, X., Gao, J., Cai, C., & Zhang, Y. (2020). LncRNA LINC01503 aggravates the progression of cervical cancer through sponging miR-342-3p to mediate FXYD3 expression. Bioscience reports, 40(6).

Xue, Y., Lai, L., Lian, W., Tu, X., Zhou, J., Dong, P., ... & Wang, Q. (2019). SOX9/FXYD3/Src Axis Is Critical for ER+ Breast Cancer Stem Cell FunctionFXYD3 Is Critical for Breast Cancer Stem Cells. Molecular Cancer Research, 17(1), 238-249.

Wang, L. J., Li, Q. J., Le, Y., Ouyang, H. Y., He, M. K., Yu, Z. S., ... & Shi, M. (2018). Prognostic significance of sodium-potassium ATPase regulator, FXYD3, in human hepatocellular carcinoma. Oncology Letters, 15(3), 3024-3030.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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