Rat Anti-FZD2 Recombinant Antibody (CBXF-1818) (CBMAB-F3353-CQ)

Name: Rat Anti-FZD2 Recombinant Antibody (CBXF-1818)
SKU: CBMAB-F3353-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBXF-1818

Application

WB, IP

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

frizzled class receptor 2

Introduction

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways.

Entrez Gene ID

57265

UniProt ID

Q9JIP6

Alternative Names

Frizzled Class Receptor 2; Frizzled 2, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 2; Fz-2; FzE2; HFz2;

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:25759469).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

(Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium (PubMed:27680706, PubMed:29748286).

TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling (PubMed:29748286).

Biological Process

Canonical Wnt signaling pathway Source: UniProtKB
Cochlea morphogenesis Source: Ensembl
Epithelial cell differentiation Source: Ensembl
Hard palate development Source: Ensembl
Inner ear receptor cell development Source: Ensembl
Membranous septum morphogenesis Source: Ensembl
Muscular septum morphogenesis Source: Ensembl
Neuron differentiation Source: UniProtKB
Outflow tract morphogenesis Source: Ensembl
Planar cell polarity pathway involved in neural tube closure Source: Ensembl
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Sensory perception of smell Source: Ensembl
Wnt signaling pathway Source: UniProtKB
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane; Membrane

Involvement in disease

Omodysplasia 2 (OMOD2):
A rare autosomal dominant skeletal dysplasia characterized by short humeri, radial head dislocation, short first metacarpals, facial dysmorphism and genitourinary anomalies.
Defects in FZD2 have been found in patients with Robinow syndrome-like features including short-limb dwarfism, broad thumbs and craniofacial abnormalities.

Topology

Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-279
Helical: 280-300
Extracellular: 301-327
Helical: 328-348
Cytoplasmic: 349-370
Helical: 371-391
Extracellular: 392-414
Helical: 415-435
Cytoplasmic: 436-461
Helical: 462-482
Extracellular: 483-519
Helical: 520-540
Cytoplasmic: 541-565

PTM

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Tuluhong, D., Chen, T., Wang, J., Zeng, H., Li, H., Dunzhu, W., ... & Wang, S. (2021). FZD2 promotes TGF-β-induced epithelial-to-mesenchymal transition in breast cancer via activating notch signaling pathway. Cancer Cell International, 21, 1-13.

Zhou, M., Sun, X., & Zhu, Y. (2021). Analysis of the role of Frizzled 2 in different cancer types. FEBS Open bio, 11(4), 1195-1208.

Xu, Y., Zhang, Q., Lin, F., Zhu, L., Huang, F., Zhao, L., & Ou, R. (2019). Casiopeina II‑gly acts on lncRNA MALAT1 by miR‑17‑5p to inhibit FZD2 expression via the Wnt signaling pathway during the treatment of cervical carcinoma. Oncology reports, 42(4), 1365-1379.

Nagasaki, K., Nishimura, G., Kikuchi, T., Nyuzuki, H., Sasaki, S., Ogawa, Y., & Saitoh, A. (2018). Nonsense mutations in FZD2 cause autosomal‐dominant omodysplasia: Robinow syndrome‐like phenotypes. American journal of medical genetics Part A, 176(3), 739-742.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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