Mouse Recombinant FZD2 protein, hFc Tag (V2LY-0526-LY8342)

Name: Mouse Recombinant FZD2 protein, hFc Tag
SKU: V2LY-0526-LY8342
Rating: 5 (1 reviews)

See all FZD2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant FZD2 protein, hFc Tag consist of Amino Acid: 29-168 and predicts a molecular mass of 44.2 kDa.

Molecule Mass

44.2 kDa

Sequence

Amino Acid: 29-168

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

frizzled class receptor 2

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:25759469).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

(Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium (PubMed:27680706, PubMed:29748286).

TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling (PubMed:29748286).

Biological Process

Canonical Wnt signaling pathway Source: UniProtKB
Cochlea morphogenesis Source: Ensembl
Epithelial cell differentiation Source: Ensembl
Hard palate development Source: Ensembl
Inner ear receptor cell development Source: Ensembl
Membranous septum morphogenesis Source: Ensembl
Muscular septum morphogenesis Source: Ensembl
Neuron differentiation Source: UniProtKB
Outflow tract morphogenesis Source: Ensembl
Planar cell polarity pathway involved in neural tube closure Source: Ensembl
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Sensory perception of smell Source: Ensembl
Wnt signaling pathway Source: UniProtKB
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane; Membrane

Involvement in disease

Omodysplasia 2 (OMOD2):
A rare autosomal dominant skeletal dysplasia characterized by short humeri, radial head dislocation, short first metacarpals, facial dysmorphism and genitourinary anomalies.
Defects in FZD2 have been found in patients with Robinow syndrome-like features including short-limb dwarfism, broad thumbs and craniofacial abnormalities.

Topology

Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-279
Helical: 280-300
Extracellular: 301-327
Helical: 328-348
Cytoplasmic: 349-370
Helical: 371-391
Extracellular: 392-414
Helical: 415-435
Cytoplasmic: 436-461
Helical: 462-482
Extracellular: 483-519
Helical: 520-540
Cytoplasmic: 541-565

PTM

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Tuluhong, D., Chen, T., Wang, J., Zeng, H., Li, H., Dunzhu, W., ... & Wang, S. (2021). FZD2 promotes TGF-β-induced epithelial-to-mesenchymal transition in breast cancer via activating notch signaling pathway. Cancer Cell International, 21, 1-13.

Zhou, M., Sun, X., & Zhu, Y. (2021). Analysis of the role of Frizzled 2 in different cancer types. FEBS Open bio, 11(4), 1195-1208.

Xu, Y., Zhang, Q., Lin, F., Zhu, L., Huang, F., Zhao, L., & Ou, R. (2019). Casiopeina II‑gly acts on lncRNA MALAT1 by miR‑17‑5p to inhibit FZD2 expression via the Wnt signaling pathway during the treatment of cervical carcinoma. Oncology reports, 42(4), 1365-1379.

Nagasaki, K., Nishimura, G., Kikuchi, T., Nyuzuki, H., Sasaki, S., Ogawa, Y., & Saitoh, A. (2018). Nonsense mutations in FZD2 cause autosomal‐dominant omodysplasia: Robinow syndrome‐like phenotypes. American journal of medical genetics Part A, 176(3), 739-742.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Recombinant FZD2 protein, hFc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-FZD2 Recombinant Antibody (CBXF-0984) (CAT#: CBMAB-F3225-CQ)

Rat Anti-FZD2 (Extracellular domain) Recombinant Antibody (CBXF-0985) (CAT#: CBMAB-F2510-CQ)

Rat Anti-FZD2 Recombinant Antibody (CBXF-2692) (CAT#: CBMAB-F0128-CQ)

Mouse Anti-FZD2 Recombinant Antibody (1E7) (CAT#: CBMAB-A3251-LY)

Rat Anti-FZD2 Recombinant Antibody (CBXF-2188) (CAT#: CBMAB-F1849-CQ)

Mouse Anti-FZD2 Recombinant Antibody (CBXF-3259) (CAT#: V2LY-1225-LY389)

Rat Anti-FZD2 Recombinant Antibody (CBXF-3261) (CAT#: CBMAB-F3955-CQ)

Mouse Anti-FZD2 Recombinant Antibody (CBXF-0983) (CAT#: CBMAB-F1008-CQ)

Rat Anti-FZD2 Recombinant Antibody (CBXF-1694) (CAT#: CBMAB-F4159-CQ)

Mouse Anti-FZD2 Recombinant Antibody (CBXF-0983) (CAT#: V2LY-1225-LY387)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry