Mouse Anti-FZR1 Recombinant Antibody (4C4) (CBMAB-F1015-CQ)

Name: Mouse Anti-FZR1 Recombinant Antibody (4C4)
SKU: CBMAB-F1015-CQ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

4C4

Application

WB, IHC-P, S-ELISA

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fizzy And Cell Division Cycle 20 Related 1

Introduction

FZR1 (Fizzy And Cell Division Cycle 20 Related 1) is a Protein Coding gene. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Innate Immune System. An important paralog of this gene is CDC20.

Entrez Gene ID

51343

UniProt ID

Q9UM11

Alternative Names

Fizzy And Cell Division Cycle 20 Related 1; Fizzy/Cell Division Cycle 20 Related 1; CDC20-Like Protein 1; Cdh1/Hct1 Homolog; CDC20 Homolog 1; HCDH1; CDH1; FZR; Fizzy/Cell Division Cycle 20 Related 1 (Drosophila);

Function

Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192).

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: UniProtKB
Cell division Source: UniProtKB-KW
DNA repair Source: UniProtKB-KW
Lens fiber cell differentiation Source: Ensembl
Mitotic G2 DNA damage checkpoint signaling Source: UniProtKB
Negative regulation of cell aging Source: Ensembl
Positive regulation of anaphase-promoting complex-dependent catabolic process Source: GO_Central
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of protein catabolic process Source: UniProtKB
Positive regulation of ubiquitin protein ligase activity Source: UniProtKB
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of meiotic nuclear division Source: Ensembl

Cellular Location

Isoform 2: Nucleus
Isoform 3: Cytoplasm

PTM

Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159; deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK.
Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex; leading to its degradation by the proteasome.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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