Mouse Anti-GALNT1 Recombinant Antibody (CBFYH-0231) (V2LY-1225-LY624)

Name: Mouse Anti-GALNT1 Recombinant Antibody (CBFYH-0231)
SKU: V2LY-1225-LY624
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GALNT1 Recombinant Antibody (CBFYH-0231) (V2LY-1225-LY624) in IF

Nuclear translocation of β-catenin examined by immunofluorescence assays in GALNT1-silenced MKN45, SGC7901, and AGS cells. Scale bar: 50μm. ...View More

Zhang, J., Wang, H., Wu, J., Yuan, C., Chen, S., Liu, S., Huo, M., Zhang, C., & He, Y. (2022). GALNT1 Enhances Malignant Phenotype of Gastric Cancer via Modulating CD44 Glycosylation to Activate the Wnt/β-catenin Signaling Pathway. International journal of biological sciences, 18(16), 6068-6083.

Mouse Anti-GALNT1 Recombinant Antibody (CBFYH-0231) (V2LY-1225-LY624) in IF

Immunofluorescence assay depicting the nuclear translocation of β-catenin in AGS cells transfected with OE-GALNT1 and shCD44. Scale bar: 50 µm. ...View More

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYH-0231

Application

WB, ELISA, IF

Immunogen

Partial recombinant corresponding to aa 42-123 from human GALNT1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
IF(ICC)	10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1)

Entrez Gene ID

2589

UniProt ID

Q10472

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.

Biological Process

O-glycan processing Source: Reactome
Protein O-linked glycosylation Source: UniProtKB
Protein O-linked glycosylation via serine Source: BHF-UCL
Protein O-linked glycosylation via threonine Source: BHF-UCL
Viral protein processing Source: Reactome

Cellular Location

Polypeptide N-acetylgalactosaminyltransferase 1: Golgi stack membrane
Polypeptide N-acetylgalactosaminyltransferase 1 soluble form: Secreted

Topology

Cytoplasmic: 1-8
Helical: 9-28
Lumenal: 29-559

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GALNT1 (AA 42-123) Recombinant Antibody (CBFYH-0231) (CAT#: CBMAB-H1102-FY)

Related Products

Mouse Anti-GALNT1 (AA 42-123) Recombinant Antibody (CBFYH-0231) (CAT#: CBMAB-H1102-FY)

Mouse Anti-GALNT1 Recombinant Antibody (3C10) (CAT#: CBMAB-G1852-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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