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Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (V2LY-1225-LY631)

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Published Data
V2LY-1225-LY631-1.png
Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (V2LY-1225-LY631) in WB
Western blot was used to detect expression of GALNT6 in pancreatic cancer cell lines and pancreatic ductal cell. ...View More
Ding, M., Liu, J., Lv, H., Zhu, Y., Chen, Y., Peng, H., Fan, S., & Chen, X. (2023). Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway. Frontiers in oncology, 13, 1097772.
V2LY-1225-LY631-2.png
Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (V2LY-1225-LY631) in WB and IP
Co-ip was performed to detect the endogenous binding of GALNT6 to CCDC88C. ...View More
Deng, B., Zhang, S., Zhou, Y., Sun, T., Zhu, Y., Fei, J., Li, A., & Miao, Y. (2024). CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription. Cancer cell international, 24(1), 237.
V2LY-1225-LY631-3.png
Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (V2LY-1225-LY631) in IF and WB
A549, H1299, SPCA-1, and PC9 cells were transduced with control lentivirus (NC) or lentivirus for GALNT6 over-expression (G6-OE) or silencing (shNC, shG6-1/2), respectively. GALNT6, E-cadherin, N-cadherin and Slug expression in individual groups of cells were examined by immunofluorescent confocal microscopy and Western blot. a, c Immunofluorescence analysis. b, d Western blot analysis. Data are representative images or present as the mean ± SD of each group from three independent experiments. **p < 0.01; ***p < 0.001. ...View More
Song, J., Liu, W., Wang, J., Hao, J., Wang, Y., You, X., Du, X., Zhou, Y., Ben, J., Zhang, X., Ye, M., & Wang, Q. (2020). GALNT6 promotes invasion and metastasis of human lung adenocarcinoma cells through O-glycosylating chaperone protein GRP78. Cell death & disease, 11(5), 352.
V2LY-1225-LY631-4.png
Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (V2LY-1225-LY631) in IF
Immunofluorescent analysis of GALNT6 (green) and GRP78 (red) expression and distribution. We observed that GALNT6 was located in the Golgi if GRP78 was not expressed (white arrows), but in the ER of GRP78 over-expressing cells, indicating that GRP78 over-expression promoted the Golgi-to-ER relocation of GALNT6 (yellow arrows). ...View More
Song, J., Liu, W., Wang, J., Hao, J., Wang, Y., You, X., Du, X., Zhou, Y., Ben, J., Zhang, X., Ye, M., & Wang, Q. (2020). GALNT6 promotes invasion and metastasis of human lung adenocarcinoma cells through O-glycosylating chaperone protein GRP78. Cell death & disease, 11(5), 352.
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Basic Information

Host Animal
Mouse
Clone
CBLG1-511
Application
WB, ELISA, IP
Immunogen
Recombinant human GalNAc-T6.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Polypeptide N-Acetylgalactosaminyltransferase 6
Entrez Gene ID
11226
UniProt ID
Q8NCL4
Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.
Biological Process
O-glycan processing Source: Reactome
Protein O-linked glycosylation Source: ProtInc
Cellular Location
Golgi apparatus membrane
Topology
Cytoplasmic: 1-8
Helical: 9-28
Lumenal: 29-622
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GALNT6 Recombinant Antibody (CBLG1-511) (CAT#: CBMAB-G1867-LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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