Mouse Anti-GAMT Recombinant Antibody (AP2451) (V2LY-1225-LY640)

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Basic Information

Host Animal
Mouse
Clone
AP2451
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-160 mapping at the N-terminus of human GAMT.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Guanidinoacetate N-Methyltransferase
Entrez Gene ID
Human2593
Mouse14431
Rat25257
UniProt ID
HumanQ14353
MouseO35969
RatP10868
Function
Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor (PubMed:26003046, PubMed:24415674, PubMed:26319512).

Important in nervous system development (PubMed:24415674).
Biological Process
Animal organ morphogenesis Source: Ensembl
Creatine biosynthetic process Source: UniProtKB
Creatine metabolic process Source: Reactome
Methylation Source: UniProtKB-KW
Muscle contraction Source: ProtInc
Regulation of multicellular organism growth Source: Ensembl
Spermatogenesis Source: Ensembl
Cellular Location
Cytosol; Nucleus; Cytoplasm
Involvement in disease
Cerebral creatine deficiency syndrome 2 (CCDS2):
An autosomal recessive disorder characterized by developmental delay and regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures, movement disturbances, severe depletion of creatine and phosphocreatine in the brain, and accumulation of guanidinoacetic acid in brain and body fluids.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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