Mouse Anti-GDF10 Recombinant Antibody (AP2483) (V2LY-1225-LY971)

Name: Mouse Anti-GDF10 Recombinant Antibody (AP2483)
SKU: V2LY-1225-LY971
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GDF10 Recombinant Antibody (AP2483) (V2LY-1225-LY971) in IF

Representative immunofluorescence staining images and quantification of the number of PCs 90 (Calbindin, green), the integrated intensity of granule cells (GCs), and the integrated intensity of 91 Bergmann glial cells (BGCs, Gdf10, red) in the cerebella. ...View More

Wang, F., Wang, Q., Liu, B., Mei, L., Ma, S., Wang, S., Wang, R., Zhang, Y., Niu, C., Xiong, Z., Zheng, Y., Zhang, Z., Shi, J., & Song, X. (2021). The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum. Cell death and differentiation, 28(9), 2634-2650.

Mouse Anti-GDF10 Recombinant Antibody (AP2483) (V2LY-1225-LY971) in WB

Protein levels of BMP3, β-catenin, and β-actin were assessed by western blot analysis. ...View More

Kokabu, S., & Rosen, V. (2017). BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling. FEBS open bio, 8(2), 168-176.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

AP2483

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 363-435 of human BMP-3.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Growth Differentiation Factor 10

Entrez Gene ID

Human	2662
Rat	79216

UniProt ID

Human	P55107
Rat	P55108

Function

Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.

Biological Process

Negative regulation of osteoblast differentiation Source: UniProtKB
Osteoblast differentiation Source: InterPro
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: UniProtKB
Skeletal system development Source: ProtInc
SMAD protein signal transduction Source: GO_Central
Transforming growth factor beta receptor signaling pathway Source: ProtInc

Cellular Location

Secreted

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-GDF10 Recombinant Antibody (1B7D12) (CAT#: CBMAB-AP2483LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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