Mouse Anti-GFER Recombinant Antibody (6C10) (CBMAB-Z0358-WJ)

Name: Mouse Anti-GFER Recombinant Antibody (6C10)
SKU: CBMAB-Z0358-WJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GFER Recombinant Antibody (6C10) (CBMAB-Z0358-WJ) in WB

Western blot analysis of the mRNA and protein levels of ALR, respectively, in U266 multiple myeloma cells and healthy peripheral blood mononuclear cells (PBMCs). ...View More

Zeng, H. Q., Luo, Y., Lou, S. F., Liu, Q., Zhang, L., & Deng, J. C. (2017). Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 50(10), e6139.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6C10

Application

WB, IP, IF, ELISA

Immunogen

Full length ALR of human.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Growth Factor, Augmenter Of Liver Regeneration

Introduction

This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Entrez Gene ID

2671

UniProt ID

P55789

Alternative Names

SCN2; GFI-1; GFI1A; ZNF163

Function

Isoform 1:
FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen.

Isoform 2:
May act as an autocrine hepatotrophic growth factor promoting liver regeneration.

Biological Process

Liver development Source: GO_Central

Cellular Location

Isoform 1: Mitochondrion intermembrane space; Mitochondrion
Isoform 2: Secreted; Cytoplasm

Involvement in disease

Myopathy, mitochondrial progressive, with congenital cataract, hearing loss and developmental delay (MPMCHD):
A disease characterized by progressive myopathy and partial combined respiratory-chain deficiency, congenital cataract, sensorineural hearing loss, and developmental delay.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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