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Mouse Anti-GFPT1 Recombinant Antibody (G3198) (V2LY-1225-LY1130)

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Published Data
V2LY-1225-LY1130-1.png
Mouse Anti-GFPT1 Recombinant Antibody (G3198) (V2LY-1225-LY1130) in Immunoblot
Protein levels for GFPT1, AR-FL and AR-V7 assessed using immunoblot analysis in GFPT1 KD 22 Rv1 cells and NT controls. GAPDH is used as loading control. ...View More
Kaushik, A. K., Shojaie, A., Panzitt, K., Sonavane, R., Venghatakrishnan, H., Manikkam, M., Zaslavsky, A., Putluri, V., Vasu, V. T., Zhang, Y., Khan, A. S., Lloyd, S., Szafran, A. T., Dasgupta, S., Bader, D. A., Stossi, F., Li, H., Samanta, S., Cao, X., Tsouko, E., … Sreekumar, A. (2016). Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer. Nature communications, 7, 11612.
V2LY-1225-LY1130-2.png
Mouse Anti-GFPT1 Recombinant Antibody (G3198) (V2LY-1225-LY1130) in Immunoblot
Decreased protein levels for GFPT1 in GNPNAT1 KD 22Rv1 and LNCaP-ABL cells assessed using immunoblot (representative blots, n=2 each). ...View More
Kaushik, A. K., Shojaie, A., Panzitt, K., Sonavane, R., Venghatakrishnan, H., Manikkam, M., Zaslavsky, A., Putluri, V., Vasu, V. T., Zhang, Y., Khan, A. S., Lloyd, S., Szafran, A. T., Dasgupta, S., Bader, D. A., Stossi, F., Li, H., Samanta, S., Cao, X., Tsouko, E., … Sreekumar, A. (2016). Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer. Nature communications, 7, 11612.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
G3198
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 226-274 mapping within an internal region of human GFAT1.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Pig
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Glutamine--Fructose-6-Phosphate Transaminase 1
Entrez Gene ID
Human2673
Rat297417
UniProt ID
HumanQ06210
RatP82808
Function
Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.
Biological Process
Circadian regulation of gene expression Source: UniProtKB
Energy reserve metabolic process Source: ProtInc
Fructose 6-phosphate metabolic process Source: GO_Central
Glutamine metabolic process Source: UniProtKB-KW
Protein N-linked glycosylation Source: GO_Central
UDP-N-acetylglucosamine biosynthetic process Source: Reactome
UDP-N-acetylglucosamine metabolic process Source: GO_Central
Cellular Location
Cytosol; Extracellular exosome
Involvement in disease
Myasthenic syndrome, congenital, 12 (CMS12):
A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness. CMS12 is characterized by onset of proximal muscle weakness in the first decade. Individuals with this condition have a recognizable pattern of weakness of shoulder and pelvic girdle muscles, and sparing of ocular or facial muscles. EMG classically shows a decremental response to repeated nerve stimulation, a sign of neuromuscular junction dysfunction. Affected individuals show a favorable response to acetylcholinesterase (AChE) inhibitors.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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