Mouse Anti-GFRA1 Recombinant Antibody (CBFYH-0335) (V2LY-1225-LY1134)

Name: Mouse Anti-GFRA1 Recombinant Antibody (CBFYH-0335)
SKU: V2LY-1225-LY1134
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GFRA1 Recombinant Antibody (CBFYH-0335) (V2LY-1225-LY1134) in IF

Effect of temperature on the development of different stages of spermatogenesis cells in vitro. Developed cells and organoids were collected BC in MCS after two to six weeks of culture at 37 °C and 35 °C and were fixed and stained with antibodies specific for cells of different stages of spermatogenesis: pre-meiotic markers (VASA and GFR-α), a meiotic marker (BOULE) and a post-meiotic marker (ACROSIN). Nucleus detection DAPI (blue) was used. Negative control (NC) was without the first antibody, BC-before culture. ...View More

Jorban, A., Lunenfeld, E., & Huleihel, M. (2024). Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro. International journal of molecular sciences, 25(4), 2160.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBFYH-0335

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 368-437 of human GFRα-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GDNF family receptor alpha 1

Entrez Gene ID

2674

UniProt ID

P56159

Function

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).

Biological Process

Cell surface receptor signaling pathway Source: UniProtKB
Nervous system development Source: GO_Central

Cellular Location

Cell membrane; Endosome; Multivesicular body; Trans-Golgi network. Localizes mainly to the plasma membrane. In the presence of SORL1, shifts to vesicular structures, including trans-Golgi network, endosomes and multivesicular bodies.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GFRA1 Recombinant Antibody (CBFYH-0335) (CAT#: CBMAB-H1204-FY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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