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Mouse Anti-GGT5 Recombinant Antibody (CBFYH-0341) (V2LY-1225-LY1183)

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Published Data
V2LY-1225-LY1183-1.png
Mouse Anti-GGT5 Recombinant Antibody (CBFYH-0341) (V2LY-1225-LY1183) in IHC
Relative expression levels of GGT5 in gastric cancer tissues and neighboring noncancerous tissues were detected by IHC (p < 0.001, n = 90). ...View More
Luo, Z., Chen, Y., Chen, B., Zhao, Z., Wu, R., & Ren, J. (2024). GGT5 facilitates migration and invasion through the induction of epithelial-mesenchymal transformation in gastric cancer. BMC medical genomics, 17(1), 82.
V2LY-1225-LY1183-2.png
Mouse Anti-GGT5 Recombinant Antibody (CBFYH-0341) (V2LY-1225-LY1183) in IHC
Transwell assays were used to detect the migration and invasion of AGS and HGC27 cells after GGT5 knockdown. *p < 0.05, **p < 0.01 and ***p < 0.001. ...View More
Luo, Z., Chen, Y., Chen, B., Zhao, Z., Wu, R., & Ren, J. (2024). GGT5 facilitates migration and invasion through the induction of epithelial-mesenchymal transformation in gastric cancer. BMC medical genomics, 17(1), 82.
Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
CBFYH-0341
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 161-395 of human GGT5.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, λ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Gamma-Glutamyltransferase 5
Entrez Gene ID
2687
UniProt ID
P36269
Function
Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione-S-conjugate such as leukotriene C4 (PubMed:21447318).

Does not cleaves gamma-glutamyl compounds such as gamma-glutamyl leucine (PubMed:21447318).

May also catalyze a transpeptidation reaction in addition to the hydrolysis reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed:21447318).

Acts as a negative regulator of geranylgeranyl glutathione bioactivity by cleaving off its gamma-glutamyl group, playing a role in adaptive immune responses (PubMed:30842656).
Biological Process
Cellular amino acid metabolic process Source: UniProtKB
Fatty acid metabolic process Source: BHF-UCL
Glutathione biosynthetic process Source: UniProtKB-KW
Glutathione catabolic process Source: UniProtKB
Inflammatory response Source: GO_Central
Leukotriene D4 biosynthetic process Source: UniProtKB
Proteolysis Source: UniProtKB
Response to estradiol Source: GO_Central
Response to lipopolysaccharide Source: GO_Central
Response to tumor necrosis factor Source: GO_Central
Spermatogenesis Source: GO_Central
Cellular Location
Membrane
Topology
Cytoplasmic: 1-8
Helical: 9-29
Extracellular: 30-586
PTM
Cleaved by autocatalysis into a large and a small subunit.
Glycosylated.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GGT5 (AA 510-586) Recombinant Antibody (CBFYH-0341) (CAT#: CBMAB-H1210-FY)

Related Products

Mouse Anti-GGT5 (AA 510-586) Recombinant Antibody (CBFYH-0341) (CAT#: CBMAB-H1210-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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