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Mouse Anti-GJB3 Recombinant Antibody (CBLG1-110) (V2LY-1225-LY1384)

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Published Data
V2LY-1225-LY1384-1.png
Mouse Anti-GJB3 Recombinant Antibody (CBLG1-110) (V2LY-1225-LY1384) in IHC
The representative pictures display the HE, GJB3 and IgG staining in human ureter tissues (U#1 and U#2, respectively). ...View More
Liu, J., Wang, X., Jiang, W., Azoitei, A., Eiseler, T., Eckstein, M., Hartmann, A., Stilgenbauer, S., Elati, M., Hohwieler, M., Kleger, A., John, A., Wezel, F., Zengerling, F., Bolenz, C., & Günes, C. (2024). Impairment of α-tubulin and F-actin interactions of GJB3 induces aneuploidy in urothelial cells and promotes bladder cancer cell invasion. Cellular & molecular biology letters, 29(1), 94.
V2LY-1225-LY1384-2.png
Mouse Anti-GJB3 Recombinant Antibody (CBLG1-110) (V2LY-1225-LY1384) in IHC
The IHC images display Gjb3 staining (red arrows) in mouse normal bladder and bladder cancer tissues during BBN-induced BC progression. ...View More
Liu, J., Wang, X., Jiang, W., Azoitei, A., Eiseler, T., Eckstein, M., Hartmann, A., Stilgenbauer, S., Elati, M., Hohwieler, M., Kleger, A., John, A., Wezel, F., Zengerling, F., Bolenz, C., & Günes, C. (2024). Impairment of α-tubulin and F-actin interactions of GJB3 induces aneuploidy in urothelial cells and promotes bladder cancer cell invasion. Cellular & molecular biology letters, 29(1), 94.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBLG1-110
Application
WB, IP, IF, ELISA
Immunogen
Recombinant human connexin 31.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
gap junction protein, beta 3, 31kDa
Entrez Gene ID
2707
UniProt ID
O75712
Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Biological Process
Cell-cell signaling Source: GO_Central
Cellular response to retinoic acid Source: Ensembl
In utero embryonic development Source: Ensembl
Placenta development Source: Ensembl
Skin development Source: Ensembl
Spermatogenesis Source: Ensembl
Cellular Location
Cell membrane; Gap junction
Involvement in disease
Erythrokeratodermia variabilis et progressiva 1 (EKVP1):
A form of erythrokeratodermia variabilis et progressiva, a genodermatosis characterized by the coexistence of two independent skin lesions: transient erythema and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases.
Deafness, autosomal dominant, 2B (DFNA2B):
A form of non-syndromic sensorineural deafness characterized by progressive high frequency hearing loss in adulthood, with milder expression in females.
Topology
Cytoplasmic: 1-20
Helical: 21-40
Extracellular: 41-75
Helical: 76-98
Cytoplasmic: 99-126
Helical: 127-149
Extracellular: 150-187
Helical: 188-210
Cytoplasmic: 211-270
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GJB3 Recombinant Antibody (CBLG1-110) (CAT#: CBMAB-G0493-LY)

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Mouse Anti-GJB3 Recombinant Antibody (CBLG1-1205) (CAT#: V2LY-1225-LY1385)

Mouse Anti-GJB3 Recombinant Antibody (CBLG1-110) (CAT#: CBMAB-G0493-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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