Mouse Anti-GLP1R Recombinant Antibody (CBFYH-3446) (V2LY-1225-LY1504)

Name: Mouse Anti-GLP1R Recombinant Antibody (CBFYH-3446)
SKU: V2LY-1225-LY1504
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GLP1R Recombinant Antibody (CBFYH-3446) (V2LY-1225-LY1504) in IF

HEK293 cells expressing the hGLP-1R were stimulated with GLP-1, compound 2 or compound B at the indicated concentration for 60min and hGLP-1R internalisation was assessed by immunofluorescence. ...View More

Thompson, A., Stephens, J. W., Bain, S. C., & Kanamarlapudi, V. (2016). Molecular Characterisation of Small Molecule Agonists Effect on the Human Glucagon Like Peptide-1 Receptor Internalisation. PloS one, 11(4), e0154229.

Mouse Anti-GLP1R Recombinant Antibody (CBFYH-3446) (V2LY-1225-LY1504) in IF

HEK293 cells expressing the hGLP-1R were stimulated with 100nM GLP-1, 10μM compound 2 or 10μM compound B for the indicated time and assessed hGLP-1R internalisation by live cell fluorescence imaging. ...View More

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-3446

Application

Immunogen

Human GLP-1R (Arg24-Ser463).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

Application	Note
FC	0.25 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

glucagon like peptide 1 receptor

Entrez Gene ID

2740

UniProt ID

P43220

Function

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:8405712, PubMed:8216285, PubMed:7517895, PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449).

Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed:8405712, PubMed:8216285, PubMed:7517895, PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449).

Plays a role in regulating insulin secretion in response to GLP-1 (By similarity).

Biological Process

Activation of adenylate cyclase activity Source: ProtInc
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
cAMP-mediated signaling Source: UniProtKB
Cell surface receptor signaling pathway Source: InterPro
Positive regulation of blood pressure Source: GO_Central
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 24-139
Helical: 140-164
Cytoplasmic: 165-175
Helical: 176-201
Extracellular: 202-227
Helical: 228-251
Cytoplasmic: 252-265
Helical: 176-201
Extracellular: 202-227
Helical: 228-251
Cytoplasmic: 252-265
Helical: 266-290
Extracellular: 291-305
Helical: 306-328
Cytoplasmic: 329-348
Helical: 349-370
Extracellular: 371-383
Helical: 384-404
Cytoplasmic: 405-463

PTM

N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GLP1R Recombinant Antibody (CBFYH-3446) (CAT#: CBMAB-H3993-FY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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