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Mouse Anti-GPX4 Recombinant Antibody (9H129) (CBMAB-G4750-LY)

This product is antibody recognizes GPX4. The antibody 9H129 immunoassay techniques such as: WB.
See all GPX4 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
9H129
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
E. coli-derived rhGPX4
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
lyophilized
Buffer
5% trehalose
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Glutathione Peroxidase 4
Introduction
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]
Entrez Gene ID
Human2879
Mouse625249
Rat29328
UniProt ID
HumanP36969
MouseO70325
RatP36970
Function
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity).

Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity).

Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (By similarity).

Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed:24439385).

The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity).

The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity).

May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity).

Required for normal sperm development and male fertility (By similarity).

Essential for maturation and survival of photoreceptor cells (By similarity).

Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).

Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism (PubMed:11115402).

Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway (By similarity).
Biological Process
Aging Source: Ensembl
Arachidonic acid metabolic process Source: UniProtKB
Chromatin organization Source: Ensembl
Glutathione metabolic process Source: Ensembl
Lipoxygenase pathway Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: Reactome
Negative regulation of ferroptosis Source: UniProtKB
Phospholipid metabolic process Source: UniProtKB
Protein polymerization Source: CAFA
Regulation of inflammatory response Source: Ensembl
Response to estradiol Source: Ensembl
Response to oxidative stress Source: UniProtKB
Spermatogenesis Source: UniProtKB
Cellular Location
Isoform Mitochondrial: Mitochondrion
Isoform Cytoplasmic: Cytoplasm
Involvement in disease
Spondylometaphyseal dysplasia, Sedaghatian type (SMDS):
A form of spondylometaphyseal dysplasia, a group of short stature disorders distinguished by abnormalities in the vertebrae and the metaphyses of the tubular bones. SMDS is a neonatal lethal form characterized by severe metaphyseal chondrodysplasia with mild limb shortening, platyspondyly, cardiac conduction defects, and central nervous system abnormalities.

Weaver, K., & Skouta, R. (2022). The selenoprotein glutathione peroxidase 4: from molecular mechanisms to novel therapeutic opportunities. Biomedicines, 10(4), 891.

Ursini, F., Travain, V. B., Cozza, G., Miotto, G., Roveri, A., Toppo, S., & Maiorino, M. (2022). A white paper on Phospholipid Hydroperoxide Glutathione Peroxidase (GPx4) forty years later. Free Radical Biology and Medicine, 188, 117-133.

Huang, J., Chen, G., Wang, J., Liu, S., & Su, J. (2022). Platycodin D regulates high glucose-induced ferroptosis of HK-2 cells through glutathione peroxidase 4 (GPX4). Bioengineered, 13(3), 6627-6637.

Xu, S., Wu, B., Zhong, B., Lin, L., Ding, Y., Jin, X., ... & Xu, D. (2021). Naringenin alleviates myocardial ischemia/reperfusion injury by regulating the nuclear factor-erythroid factor 2-related factor 2 (Nrf2)/System xc-/glutathione peroxidase 4 (GPX4) axis to inhibit ferroptosis. Bioengineered, 12(2), 10924-10934.

Zhang, X., Sui, S., Wang, L., Li, H., Zhang, L., Xu, S., & Zheng, X. (2020). Inhibition of tumor propellant glutathione peroxidase 4 induces ferroptosis in cancer cells and enhances anticancer effect of cisplatin. Journal of cellular physiology, 235(4), 3425-3437.

Wei, Y., Lv, H., Shaikh, A. B., Han, W., Hou, H., Zhang, Z., ... & Shang, P. (2020). Directly targeting glutathione peroxidase 4 may be more effective than disrupting glutathione on ferroptosis-based cancer therapy. Biochimica et Biophysica Acta (BBA)-General Subjects, 1864(4), 129539.

Zhang, Z., Wu, Y. U., Yuan, S., Zhang, P., Zhang, J., Li, H., ... & Chen, G. (2018). Glutathione peroxidase 4 participates in secondary brain injury through mediating ferroptosis in a rat model of intracerebral hemorrhage. Brain research, 1701, 112-125.

Kinowaki, Y., Kurata, M., Ishibashi, S., Ikeda, M., Tatsuzawa, A., Yamamoto, M., ... & Yamamoto, K. (2018). Glutathione peroxidase 4 overexpression inhibits ROS-induced cell death in diffuse large B-cell lymphoma. Laboratory Investigation, 98(5), 609-619.

Li, C., Deng, X., Xie, X., Liu, Y., Friedmann Angeli, J. P., & Lai, L. (2018). Activation of glutathione peroxidase 4 as a novel anti-inflammatory strategy. Frontiers in Pharmacology, 9, 1120.

Li, C., Deng, X., Zhang, W., Xie, X., Conrad, M., Liu, Y., ... & Lai, L. (2018). Novel allosteric activators for ferroptosis regulator glutathione peroxidase 4. Journal of medicinal chemistry, 62(1), 266-275.

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For research use only. Not intended for any clinical use.

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