Mouse Anti-GYPA Recombinant Antibody (NaM10-6G4) (CBMAB-G5838-LY)

Name: Mouse Anti-GYPA Recombinant Antibody (NaM10-6G4)
SKU: CBMAB-G5838-LY
Rating: 5 (1 reviews)

See all GYPA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

NaM10-6G4

Application

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Glycophorin A (MNS Blood Group)

Introduction

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Entrez Gene ID

2993

UniProt ID

P02724

Alternative Names

Glycophorin A (MNS Blood Group); Glycophorin A (MN Blood Group); Sialoglycoprotein Alpha; MN Sialoglycoprotein; PAS-2; GPA; Erythroid-Lineage-Specific Membrane Sialoglycoprotein; Recombinant Glycophorin A-B Miltenberger-DR; Glycophorin A (Includes MN Blood Group); Mi.V Glycoprotein (24 AA); Glycophorin Sta Type C; Glycophorin A; GPA; Glycophorin Erik; Glycophorin MiI;

Function

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Cellular Location

Cell membrane. Appears to be colocalized with SLC4A1.

Topology

Extracellular: 20-91
Helical: 92-114
Cytoplasmic: 115-150

PTM

The major O-linked glycan are NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH (about 78 %) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH NeuAc-alpha-2-8-NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta-3-1-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

Suwanwootichai, P., Lopez, G. H., Emthip, M., Wilson, B., Millard, G. M., Onpuns, S., ... & Liew, Y. W. (2022). Fatal haemolytic transfusion reaction due to anti‐Ena and identification of a novel GYPA c. 295delG variant in a Thai family. Vox Sanguinis, 117(11), 1327-1331.

Liang, Y., Ren, J., Zhong, F., Hong, W., Su, Y., Wu, F., ... & Xu, Y. (2022). Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors. Vox Sanguinis, 117(5), 715-723.

Oda, A., Suzuki, Y., Isa, K., Ogasawara, K., Yabe, R., Kimura, T., ... & Tsuno, N. H. (2021). GP. MOT: A novel glycophorin variant identified in a Japanese blood donor. Transfusion, 61(10), 2825-2829.

Ito, S., Kaito, S., Miyazaki, T., Kikuchi, G., Isa, K., Tsuneyama, H., ... & Satake, M. (2020). A new antigen SUMI carried on glycophorin A encoded by the GYPA* M with c. 91A> C (p. Thr31Pro) belongs to the MNS blood group system. Transfusion, 60(6), 1287-1293.

Bigham, A. W., Magnaye, K., Dunn, D. M., Weiss, R. B., & Bamshad, M. (2018). Complex signatures of natural selection at GYPA. Human genetics, 137, 151-160.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-GYPA Recombinant Antibody (NaM10-6G4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-GYPA Recombinant Antibody (CB229) (CAT#: CBMAB-CS229LY)

Mouse Anti-GYPA Recombinant Antibody (CBWJC-3752) (CAT#: CBMAB-C5017WJ)

Mouse Anti-GYPA Recombinant Antibody (13H262) (CAT#: CBMAB-G5829-LY)

Mouse Anti-GYPA Recombinant Antibody (CBFYH-0546) (CAT#: CBMAB-H1430-FY)

Mouse Anti-GYPA Recombinant Antibody (13H263) (CAT#: CBMAB-G0943-LY)

Mouse Anti-GYPA Monoclonal Antibody (CBFYH-2992) (CAT#: CBMAB-H0142-FY)

Mouse Anti-GYPA Recombinant Antibody (143-13) (CAT#: CBMAB-G5830-LY)

Mouse Anti-GYPA Recombinant Antibody (CB228) (CAT#: CBMAB-CS228LY)

Mouse Anti-GYPA Recombinant Antibody (GA-R2) (CAT#: CBMAB-H1426-FY)

Mouse Anti-GYPA Recombinant Antibody (CBFYH-0550) (CAT#: CBMAB-H0263-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry