Rabbit Anti-GYPA Recombinant Antibody (
27A8) (V2LY-0725-LY1124)

Name: Rabbit Anti-GYPA Recombinant Antibody (27A8)
SKU: V2LY-0725-LY1124
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-GYPA Recombinant Antibody (27A8) (V2LY-0725-LY1124) in FC

Overlay Peak curve showing K562 cells surface stained with V2LY-0725-LY1124 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

27A8

Application

ELISA, FC

Immunogen

A synthesized peptide derived from Human GYPA.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Glycophorin A (MNS Blood Group)

Entrez Gene ID

2993

UniProt ID

P02724

Function

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Cellular Location

Cell membrane. Appears to be colocalized with SLC4A1.

Topology

Extracellular: 20-91
Helical: 92-114
Cytoplasmic: 115-150

PTM

The major O-linked glycan are NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH (about 78 %) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH NeuAc-alpha-2-8-NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta-3-1-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

Suwanwootichai, P., Lopez, G. H., Emthip, M., Wilson, B., Millard, G. M., Onpuns, S., ... & Liew, Y. W. (2022). Fatal haemolytic transfusion reaction due to anti‐Ena and identification of a novel GYPA c. 295delG variant in a Thai family. Vox Sanguinis, 117(11), 1327-1331.

Liang, Y., Ren, J., Zhong, F., Hong, W., Su, Y., Wu, F., ... & Xu, Y. (2022). Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors. Vox Sanguinis, 117(5), 715-723.

Oda, A., Suzuki, Y., Isa, K., Ogasawara, K., Yabe, R., Kimura, T., ... & Tsuno, N. H. (2021). GP. MOT: A novel glycophorin variant identified in a Japanese blood donor. Transfusion, 61(10), 2825-2829.

Ito, S., Kaito, S., Miyazaki, T., Kikuchi, G., Isa, K., Tsuneyama, H., ... & Satake, M. (2020). A new antigen SUMI carried on glycophorin A encoded by the GYPA* M with c. 91A> C (p. Thr31Pro) belongs to the MNS blood group system. Transfusion, 60(6), 1287-1293.

Bigham, A. W., Magnaye, K., Dunn, D. M., Weiss, R. B., & Bamshad, M. (2018). Complex signatures of natural selection at GYPA. Human genetics, 137, 151-160.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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