Human Recombinant GYPA protein, His Tag (V2LY-0526-LY4338)

Name: Human Recombinant GYPA protein, His Tag
SKU: V2LY-0526-LY4338
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GYPA protein, His Tag consist of Amino Acid: 1-91 and predicts a molecular mass of 9.4 kDa.

Molecule Mass

9.4 kDa

Verified

HPLC

Sequence

Amino Acid: 1-91

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Glycophorin A (MNS Blood Group)

Function

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Cellular Location

Cell membrane. Appears to be colocalized with SLC4A1.

Topology

Extracellular: 20-91
Helical: 92-114
Cytoplasmic: 115-150

PTM

The major O-linked glycan are NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH (about 78 %) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-2-3-Gal-beta-1-3-[NeuAc-alpha-2-6]-GalNAcOH NeuAc-alpha-2-8-NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta-3-1-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-2-3-Gal-beta-1-3-GalNAcOH-6-1-GlcNAc-beta-4-1-[Fuc-alpha-1-2]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

Suwanwootichai, P., Lopez, G. H., Emthip, M., Wilson, B., Millard, G. M., Onpuns, S., ... & Liew, Y. W. (2022). Fatal haemolytic transfusion reaction due to anti‐Ena and identification of a novel GYPA c. 295delG variant in a Thai family. Vox Sanguinis, 117(11), 1327-1331.

Liang, Y., Ren, J., Zhong, F., Hong, W., Su, Y., Wu, F., ... & Xu, Y. (2022). Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors. Vox Sanguinis, 117(5), 715-723.

Oda, A., Suzuki, Y., Isa, K., Ogasawara, K., Yabe, R., Kimura, T., ... & Tsuno, N. H. (2021). GP. MOT: A novel glycophorin variant identified in a Japanese blood donor. Transfusion, 61(10), 2825-2829.

Ito, S., Kaito, S., Miyazaki, T., Kikuchi, G., Isa, K., Tsuneyama, H., ... & Satake, M. (2020). A new antigen SUMI carried on glycophorin A encoded by the GYPA* M with c. 91A> C (p. Thr31Pro) belongs to the MNS blood group system. Transfusion, 60(6), 1287-1293.

Bigham, A. W., Magnaye, K., Dunn, D. M., Weiss, R. B., & Bamshad, M. (2018). Complex signatures of natural selection at GYPA. Human genetics, 137, 151-160.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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