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Mouse Anti-HACD3 Recombinant Antibody (5B5) (CBMAB-P0373-YC)

Provided herein is a Mouse monoclonal antibody against Human 3-Hydroxyacyl-CoA Dehydratase 3. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all HACD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5B5
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
PTPLAD1 (NP_057479, 1-113 aa) partial recombinant protein with GST tag. Immunogen sequence: MENQVLTPHV YWAQRHRELY LRVELSDVQN PAISITENVL HFKAQGHGAK GDNVYEFHLE FLDLVKPEPV YKLTQRQVNI TVQKKVSQWW ERLTKQEKRP LFLAPDFDRW LDE
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-113

Target

Full Name
3-Hydroxyacyl-CoA Dehydratase 3
Introduction
HACD3 (3-Hydroxyacyl-CoA Dehydratase 3) is a protein coding gene. Among its related pathways are Fatty Acyl-CoA Biosynthesis and Fatty acid elongation. Gene Ontology annotations related to this gene include enzyme binding and lyase activity. An important paralog of the gene is HACD4.
Entrez Gene ID
51495
UniProt ID
Q9P035
Alternative Names
3-Hydroxyacyl-CoA Dehydratase 3; Protein-Tyrosine Phosphatase-Like A Domain-Containing Protein 1; Protein Tyrosine Phosphatase-Like A Domain Containing 1; Very-Long-Chain (3R)-3-Hydroxyacyl-CoA Dehydratase 3; Butyrate-Induced Protein 1; PTPLAD1; B-IND1; BIND1;
Function
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be involved in Rac1-signaling pathways leading to the modulation of gene expression. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed:25687571).
Biological Process
Fatty acid elongation Source: UniProtKB
I-kappaB kinase/NF-kappaB signaling Source: ProtInc
JNK cascade Source: Ensembl
Positive regulation by virus of viral protein levels in host cell Source: AgBase
Positive regulation of viral genome replication Source: AgBase
Rac protein signal transduction Source: UniProtKB
Rho protein signal transduction Source: Ensembl
Small GTPase mediated signal transduction Source: ProtInc
Sphingolipid biosynthetic process Source: GO_Central
Very long-chain fatty acid biosynthetic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-149
Helical: 150-170
Lumenal: 171-185
Helical: 186-207
Cytoplasmic: 208-217
Helical: 218-235
Lumenal: 236-241
Helical: 242-256
Cytoplasmic: 257-279
Helical: 280-298
Lumenal: 299-322
Helical: 323-343
Cytoplasmic: 344-362
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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