Rat Anti-HCAR2 Monoclonal Antibody (CBFYH-3025) (CBMAB-H0254-FY)

Name: Rat Anti-HCAR2 Monoclonal Antibody (CBFYH-3025)
SKU: CBMAB-H0254-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBFYH-3025

Application

WB, FC

Immunogen

Human NIACR1

Specificity

Human

Antibody Isotype

IgG2

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Hydroxycarboxylic Acid Receptor 2

Introduction

HCAR2 (Hydroxycarboxylic Acid Receptor 2) is a Protein Coding gene. Among its related pathways are Peptide ligand-binding receptors and cAMP signaling pathway. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and nicotinic acid receptor activity. An important paralog of this gene is HCAR3.

Entrez Gene ID

338442

UniProt ID

Q8TDS4

Alternative Names

Hydroxycarboxylic Acid Receptor 2; Niacin Receptor 1; G-Protein Coupled Receptor HM74A; G-Protein Coupled Receptor 109A; Nicotinic Acid Receptor; GPR109A; NIACR1; HM74a

Function

Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid> acifran> 5-methyl nicotinic acid = acipimox>> nicotinuric acid = nicotinamide.

Biological Process

Negative regulation of lipid catabolic process Source: UniProtKB
Neutrophil apoptotic process Source: UniProtKB
Positive regulation of adiponectin secretion Source: UniProtKB
Positive regulation of neutrophil apoptotic process Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-63
Helical: 64-84
Extracellular: 85-102
Helical: 103-123
Cytoplasmic: 124-142
Helical: 143-163
Extracellular: 164-192
Helical: 193-213
Cytoplasmic: 214-229
Helical: 230-250
Extracellular: 251-273
Helical: 274-294
Cytoplasmic: 295-363

Perrone, M., Pagano, M., Belardo, C., Ricciardi, F., Ricciardi, F., Fusco, A., ... & Guida, F. (2023). Potential role of the hydroxyl carboxylic acid receptor type 2 (HCAR2) in microglia pathophysiology: A possible cross-talk with CXC chemokine receptor 1 (CXCR1). Neuropharmacology, 109456.

Pan, X., Ye, F., Ning, P., Zhang, Z., Zhang, B., Chen, G., ... & Du, Y. (2023). Structural insights into ligand recognition and selectivity of the human hydroxycarboxylic acid receptor HCAR2. bioRxiv, 2023-03.

Moutinho, M., Puntambekar, S. S., Tsai, A. P., Coronel, I., Lin, P. B., Casali, B. T., ... & Landreth, G. E. (2022). The niacin receptor HCAR2 modulates microglial response and limits disease progression in a mouse model of Alzheimer’s disease. Science translational medicine, 14(637), eabl7634.

de Souza, A. G., Lopes, I. S., Filho, A. J. M. C., Cavalcante, T. M. B., Oliveira, J. V. S., de Carvalho, M. A. J., ... & de França Fonteles, M. M. (2022). Neuroprotective effects of dimethyl fumarate against depression-like behaviors via astrocytes and microglia modulation in mice: possible involvement of the HCAR2/Nrf2 signaling pathway. Naunyn-Schmiedeberg's Archives of Pharmacology, 395(9), 1029-1045.

Abdelrahman, A. A., Powell, F. L., Jadeja, R. N., Jones, M. A., Thounaojam, M. C., Bartoli, M., ... & Martin, P. M. (2022). Expression and activation of the ketone body receptor HCAR2/GPR109A promotes preservation of retinal endothelial cell barrier function. Experimental eye research, 221, 109129.

Powell, F. L., Tawfik, A., Arjunan, P., Chopra, D. G., Al-Shabrawey, M., Singh, N., ... & Martin, P. M. (2021). Hydroxycarboxylic acid receptor 2 (HCAR2/GPR109A) expression and signaling promotes the maintenance of an immunoinhibitory retinal environment. bioRxiv, 2021-03.

Moutinho, M., Tsai, A. P. Y., Puntambekar, S. S., Casali, B. T., Lin, P. B. C., Patel, J., ... & Landreth, G. E. (2021). The role of microglia niacin receptor (HCAR2) in Alzheimer's disease. Alzheimer's & Dementia, 17, e052716.

Tuteja, S. (2019). Activation of HCAR2 by niacin: benefits beyond lipid lowering. Pharmacogenomics, 20(16), 1143-1150.

Jadeja, R. N., Jones, M. A., Fromal, O., Powell, F. L., Khurana, S., Singh, N., & Martin, P. M. (2019). Loss of GPR109A/HCAR2 induces aging-associated hepatic steatosis. Aging (Albany NY), 11(2), 386.

Chen, Y., Ouyang, X., Hoque, R., Garcia-Martinez, I., Yousaf, M. N., Tonack, S., ... & Mehal, W. Z. (2018). β-Hydroxybutyrate protects from alcohol-induced liver injury via a Hcar2-cAMP dependent pathway. Journal of hepatology, 69(3), 687-696.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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