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Rat Anti-HCAR2 Recombinant Antibody (CBWJN-0226) (CBMAB-N0869-WJ)

This product is a Rat antibody that recognizes HCAR2. The antibody CBWJN-0226 can be used for immunoassay techniques such as: FC, WB.
See all HCAR2 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
CBWJN-0226
Antibody Isotype
IgG2
Application
FC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Hydroxycarboxylic Acid Receptor 2
Introduction
HCAR2 (Hydroxycarboxylic Acid Receptor 2) is a Protein Coding gene. Among its related pathways are Peptide ligand-binding receptors and cAMP signaling pathway. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and nicotinic acid receptor activity. An important paralog of this gene is HCAR3.
Entrez Gene ID
338442
UniProt ID
Q8TDS4
Alternative Names
Hydroxycarboxylic Acid Receptor 2; Niacin Receptor 1; G-Protein Coupled Receptor HM74A; G-Protein Coupled Receptor 109A; Nicotinic Acid Receptor; GPR109A; NIACR1; HM74a;
Function
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid> acifran> 5-methyl nicotinic acid = acipimox>> nicotinuric acid = nicotinamide.
Biological Process
Negative regulation of lipid catabolic process Source: UniProtKB
Neutrophil apoptotic process Source: UniProtKB
Positive regulation of adiponectin secretion Source: UniProtKB
Positive regulation of neutrophil apoptotic process Source: UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-63
Helical: 64-84
Extracellular: 85-102
Helical: 103-123
Cytoplasmic: 124-142
Helical: 143-163
Extracellular: 164-192
Helical: 193-213
Cytoplasmic: 214-229
Helical: 230-250
Extracellular: 251-273
Helical: 274-294
Cytoplasmic: 295-363

Perrone, M., Pagano, M., Belardo, C., Ricciardi, F., Ricciardi, F., Fusco, A., ... & Guida, F. (2023). Potential role of the hydroxyl carboxylic acid receptor type 2 (HCAR2) in microglia pathophysiology: A possible cross-talk with CXC chemokine receptor 1 (CXCR1). Neuropharmacology, 109456.

Pan, X., Ye, F., Ning, P., Zhang, Z., Zhang, B., Chen, G., ... & Du, Y. (2023). Structural insights into ligand recognition and selectivity of the human hydroxycarboxylic acid receptor HCAR2. bioRxiv, 2023-03.

Moutinho, M., Puntambekar, S. S., Tsai, A. P., Coronel, I., Lin, P. B., Casali, B. T., ... & Landreth, G. E. (2022). The niacin receptor HCAR2 modulates microglial response and limits disease progression in a mouse model of Alzheimer’s disease. Science translational medicine, 14(637), eabl7634.

de Souza, A. G., Lopes, I. S., Filho, A. J. M. C., Cavalcante, T. M. B., Oliveira, J. V. S., de Carvalho, M. A. J., ... & de França Fonteles, M. M. (2022). Neuroprotective effects of dimethyl fumarate against depression-like behaviors via astrocytes and microglia modulation in mice: possible involvement of the HCAR2/Nrf2 signaling pathway. Naunyn-Schmiedeberg's Archives of Pharmacology, 395(9), 1029-1045.

Abdelrahman, A. A., Powell, F. L., Jadeja, R. N., Jones, M. A., Thounaojam, M. C., Bartoli, M., ... & Martin, P. M. (2022). Expression and activation of the ketone body receptor HCAR2/GPR109A promotes preservation of retinal endothelial cell barrier function. Experimental eye research, 221, 109129.

Powell, F. L., Tawfik, A., Arjunan, P., Chopra, D. G., Al-Shabrawey, M., Singh, N., ... & Martin, P. M. (2021). Hydroxycarboxylic acid receptor 2 (HCAR2/GPR109A) expression and signaling promotes the maintenance of an immunoinhibitory retinal environment. bioRxiv, 2021-03.

Moutinho, M., Tsai, A. P. Y., Puntambekar, S. S., Casali, B. T., Lin, P. B. C., Patel, J., ... & Landreth, G. E. (2021). The role of microglia niacin receptor (HCAR2) in Alzheimer's disease. Alzheimer's & Dementia, 17, e052716.

Tuteja, S. (2019). Activation of HCAR2 by niacin: benefits beyond lipid lowering. Pharmacogenomics, 20(16), 1143-1150.

Jadeja, R. N., Jones, M. A., Fromal, O., Powell, F. L., Khurana, S., Singh, N., & Martin, P. M. (2019). Loss of GPR109A/HCAR2 induces aging-associated hepatic steatosis. Aging (Albany NY), 11(2), 386.

Chen, Y., Ouyang, X., Hoque, R., Garcia-Martinez, I., Yousaf, M. N., Tonack, S., ... & Mehal, W. Z. (2018). β-Hydroxybutyrate protects from alcohol-induced liver injury via a Hcar2-cAMP dependent pathway. Journal of hepatology, 69(3), 687-696.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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