Rabbit Anti-HCLS1 Recombinant Antibody (D5A9) (CBMAB-CP1064-LY)

Name: Rabbit Anti-HCLS1 Recombinant Antibody (D5A9)
SKU: CBMAB-CP1064-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D5A9

Application

WB, IP, IHC-P, IF, IF (ICC), FC

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu310 of mouse HS1.

Specificity

Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

hematopoietic cell-specific Lyn substrate 1

Entrez Gene ID

Human	15163
Rat	288077

UniProt ID

Human	P49710
Rat	Q68FX4

Function

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Biological Process

Actin filament polymerization Source: InterPro
Cellular response to cytokine stimulus Source: BHF-UCL
Erythrocyte differentiation Source: UniProtKB
Intracellular signal transduction Source: ProtInc
Negative regulation of leukocyte apoptotic process Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of actin cytoskeleton reorganization Source: BHF-UCL
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of granulocyte differentiation Source: BHF-UCL
Positive regulation of macrophage differentiation Source: Ensembl
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Positive regulation of phosphatidylinositol 3-kinase signaling Source: BHF-UCL
Positive regulation of protein import into nucleus Source: BHF-UCL
Positive regulation of protein kinase B signaling Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
Regulation of actin filament polymerization Source: BHF-UCL
Regulation of transcription, DNA-templated Source: ProtInc
Response to hormone Source: UniProtKB

Cellular Location

Mitochondrion; Cytoplasm; Membrane

PTM

Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.

Quatrana, A., Morini, E., Tiano, F., Vancheri, C., Panarello, L., Romano, S., ... & Malisan, F. (2022). Hsa-miR223-3p circulating level is upregulated in Friedreich’s ataxia and inversely associated with HCLS1 associated protein X-1, HAX-1. Human Molecular Genetics, 31(12), 2010-2022.

Sircar, R., & Belbin, T. J. (2021). Adiponectin receptor2 and HCLS1 associated proteinX-1 levels are altered in postmortem schizophrenic brain. Meta Gene, 27, 100834.

Castro-Ochoa, K. F., Guerrero-Fonseca, I. M., & Schnoor, M. (2019). Hematopoietic cell-specific lyn substrate (HCLS1 or HS1): A versatile actin-binding protein in leukocytes. Journal of Leukocyte Biology, 105(5), 881-890.

Aslam, Y., Williamson, J., Romashova, V., Elder, E., Krishna, B., Wills, M., ... & Poole, E. (2019). Human cytomegalovirus upregulates expression of HCLS1 resulting in increased cell motility and transendothelial migration during latency. IScience, 20, 60-72.

Li, Y. L., Cai, W. F., Wang, L., Liu, G. S., Paul, C., Jiang, L., ... & Wu, S. Z. (2018). Identification of the functional autophagy-regulatory domain in HCLS1-associated protein X-1 that resists against oxidative stress. DNA and Cell Biology, 37(5), 432-441.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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