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Mouse Anti-HEPH Recombinant Antibody (2D3) (CBMAB-A3823-LY)

The product is antibody recognizes HEPH. The antibody 2D3 immunoassay techniques such as: WB, ELISA.
See all HEPH antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2D3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
HEPH (NP_620074, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
hephaestin
Introduction
The protein encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Entrez Gene ID
9843
UniProt ID
Q9BQS7
Alternative Names
CPL; KIAA0698
Function
May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
Biological Process
Cellular iron ion homeostasis Source: InterPro
Copper ion transport Source: UniProtKB-KW
Iron ion homeostasis Source: GO_Central
Iron ion transport Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 24-1110
Helical: 1111-1131
Cytoplasmic: 1132-1158

Ramavath, H. N., Konda, V., & Pullakhandam, R. (2023). Quercetin Inhibits Hephaestin Expression and Iron Transport in Intestinal Cells: Possible Role of PI3K Pathway. Nutrients, 15(5), 1205.

Ramavath, H. N., Mashurabad, P. C., Yaduvanshi, P. S., Veleri, S., Sharp, P. A., & Pullakhandam, R. (2022). Zinc induces hephaestin expression via a PI3K-CDX2 dependent mechanism to regulate iron transport in intestinal Caco-2 cells. Biochemical and Biophysical Research Communications, 626, 1-7.

Zacchi, P., Belmonte, B., Mangogna, A., Morello, G., Scola, L., Martorana, A., & Borelli, V. (2021). The ferroxidase hephaestin in lung cancer: pathological significance and prognostic value. Frontiers in oncology, 11, 638856.

Dlouhy, A. C., Bailey, D. K., Steimle, B. L., Parker, H. V., & Kosman, D. J. (2019). Fluorescence resonance energy transfer links membrane ferroportin, hephaestin but not ferroportin, amyloid precursor protein complex with iron efflux. Journal of Biological Chemistry, 294(11), 4202-4214.

Fuqua, B. K., Lu, Y., Frazer, D. M., Darshan, D., Wilkins, S. J., Dunn, L., ... & Anderson, G. J. (2018). Severe iron metabolism defects in mice with double knockout of the multicopper ferroxidases hephaestin and ceruloplasmin. Cellular and molecular gastroenterology and hepatology, 6(4), 405-427.

Zheng, J., Chen, M., Liu, G., Xu, E., & Chen, H. (2018). Ablation of hephaestin and ceruloplasmin results in iron accumulation in adipocytes and type 2 diabetes. FEBS letters, 592(3), 394-401.

Chen, M., Zheng, J., Liu, G., Xu, E., Wang, J., Fuqua, B. K., ... & Chen, H. (2018). Ceruloplasmin and hephaestin jointly protect the exocrine pancreas against oxidative damage by facilitating iron efflux. Redox Biology, 17, 432-439.

Xu, E., Chen, M., Zheng, J., Maimaitiming, Z., Zhong, T., & Chen, H. (2018). Deletion of hephaestin and ceruloplasmin induces a serious systemic iron deficiency and disrupts iron homeostasis. Biochemical and biophysical research communications, 503(3), 1905-1910.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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