Human HEPH ELISA Kit (V2LY-0626-LY3406)

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Tested Data
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Basic Information

Sensitivity
0.054 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
130.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
hephaestin
Function
May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
Biological Process
Cellular iron ion homeostasis Source: InterPro
Copper ion transport Source: UniProtKB-KW
Iron ion homeostasis Source: GO_Central
Iron ion transport Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 24-1110
Helical: 1111-1131
Cytoplasmic: 1132-1158

Ramavath, H. N., Konda, V., & Pullakhandam, R. (2023). Quercetin Inhibits Hephaestin Expression and Iron Transport in Intestinal Cells: Possible Role of PI3K Pathway. Nutrients, 15(5), 1205.

Ramavath, H. N., Mashurabad, P. C., Yaduvanshi, P. S., Veleri, S., Sharp, P. A., & Pullakhandam, R. (2022). Zinc induces hephaestin expression via a PI3K-CDX2 dependent mechanism to regulate iron transport in intestinal Caco-2 cells. Biochemical and Biophysical Research Communications, 626, 1-7.

Zacchi, P., Belmonte, B., Mangogna, A., Morello, G., Scola, L., Martorana, A., & Borelli, V. (2021). The ferroxidase hephaestin in lung cancer: pathological significance and prognostic value. Frontiers in oncology, 11, 638856.

Dlouhy, A. C., Bailey, D. K., Steimle, B. L., Parker, H. V., & Kosman, D. J. (2019). Fluorescence resonance energy transfer links membrane ferroportin, hephaestin but not ferroportin, amyloid precursor protein complex with iron efflux. Journal of Biological Chemistry, 294(11), 4202-4214.

Fuqua, B. K., Lu, Y., Frazer, D. M., Darshan, D., Wilkins, S. J., Dunn, L., ... & Anderson, G. J. (2018). Severe iron metabolism defects in mice with double knockout of the multicopper ferroxidases hephaestin and ceruloplasmin. Cellular and molecular gastroenterology and hepatology, 6(4), 405-427.

Zheng, J., Chen, M., Liu, G., Xu, E., & Chen, H. (2018). Ablation of hephaestin and ceruloplasmin results in iron accumulation in adipocytes and type 2 diabetes. FEBS letters, 592(3), 394-401.

Chen, M., Zheng, J., Liu, G., Xu, E., Wang, J., Fuqua, B. K., ... & Chen, H. (2018). Ceruloplasmin and hephaestin jointly protect the exocrine pancreas against oxidative damage by facilitating iron efflux. Redox Biology, 17, 432-439.

Xu, E., Chen, M., Zheng, J., Maimaitiming, Z., Zhong, T., & Chen, H. (2018). Deletion of hephaestin and ceruloplasmin induces a serious systemic iron deficiency and disrupts iron homeostasis. Biochemical and biophysical research communications, 503(3), 1905-1910.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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